https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 Serum methylarginines and spirometry-measured lung function in older adults https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:14963 Wed 11 Apr 2018 17:03:18 AEST ]]> Transsulfuration pathway thiols and methylated arginines: the hunter community study https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:14939 Wed 11 Apr 2018 15:21:27 AEST ]]> Serum thiols and cardiovascular risk scores: a combined assessment of transsulfuration pathway components and substrate/product ratios https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:14826 Wed 11 Apr 2018 09:25:23 AEST ]]> Asymmetric dimethylarginine: a possible link between vascular disease and dementia https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:23647 Thu 13 Oct 2016 11:33:45 AEDT ]]> Memory impairment is associated with serum methylarginines in older adults https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:17876 Sat 24 Mar 2018 07:56:43 AEDT ]]> Serum methylarginines and incident depression in a cohort of older adults https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:19394 Sat 24 Mar 2018 07:52:05 AEDT ]]> Targeted arginine metabolomics: a rapid, simple UPLC-QToF-MS<sup>E</sup> based approach for assessing the involvement of arginine metabolism in human disease https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:28420 G-monomethyl-L-arginine (MMA), NG,. NG-dimethyl-L-arginine (ADMA) and NG,. NG'-dimethyl-L-arginine (SDMA), all of which have the capacity to alter NOS activity. Simultaneous assessment of these analytes, when assessing the impact of arginine metabolism in human disease states, is desirable. Methods: Analytes (ARG, ADMA, SDMA, MMA, HMA, CIT and ORN) were isolated from human plasma by solvent extraction, evaporated and reconstituted. Ultra-performance liquid chromatography (UPLC) was performed on a 150mm×2.1mm T3 HSS column using a gradient mobile phase comprising ammonium formate (10mM, pH3.8) in methanol (1% to 63%). Analytes were detected by time-of-flight mass spectrometry (Q-ToF-MS) in positive ion mode with electrospray ionisation (ESI+). Data were collected using MSE. Results: Solvent extraction provided high recovery (>95%). UPLC-QToF-MSE facilitated the separation and quantification of the 7 analytes in an analysis time of 6min. The approach has high sensitivity; LOQ range from 0.005µM (NMMA) to 0.25µM (ARG and ORN), and good precision; intra- and inter-day %RSD are <6% for all analytes. Conclusions: This approach provides the capacity to quantify 7 key compounds involved in ARG metabolism in a small sample volume, with a short total analysis time. These characteristics make this approach ideal for undertaking a comprehensive characterisation of this pathway in large data sets (e.g. population studies).]]> Sat 24 Mar 2018 07:29:06 AEDT ]]>