/manager/Index ${session.getAttribute("locale")} 5 Extraction of flavonoids from bitter melon /manager/Repository/uon:19674 Wed 11 Apr 2018 11:01:38 AEST ]]> An improved solvent extraction method for the analysis of catechins and caffeine in green tea /manager/Repository/uon:17131 Sat 24 Mar 2018 08:02:30 AEDT ]]> Gut microbes modulate bioaccessibility of lead in soil /manager/Repository/uon:39212 Lactobacillus acidophilus, Lactobacillus rhamnosus and Escherichia coli. Lead toxicity to these three microbes was also examined at various pH values. Bioaccessibility of Pb was measured using gastric and intestinal extractions. Both Pb spiked and Pb-contaminated shooting range field soils were used to measure Pb bioaccessibility in the presence and absence of gut microbes. The results indicated that Pb toxicity to gut microbes, as measured by LD50 value, decreased with increasing pH, and was higher for Lactobacillus species. Gut microbes decreased the bioaccessible Pb; the effect was more pronounced at low pH, mimicking gastric conditions than in conditions closer to the intestine. Lead adsorption by these microbes increased at the higher pH tested, and E. coli adsorbed higher amounts of Pb than did the Lactobacillus species. The effect of gut microbes on reducing Pb bioaccessibility may be attributed to microbially-induced immobilization of Pb through adsorption, precipitation, and complexation reactions. The study demonstrates that bioaccessibility and subsequently bioavailability of metal(loid)s can be modulated by gut microbes, and it is important to undertake bioaccessibility measurements in the presence of gut microbes.]]> Fri 27 May 2022 11:09:22 AEST ]]>