https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 The DNA methylation of FOXO3 and TP53 as a blood biomarker of late-onset asthma https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:42668 Wed 31 Aug 2022 16:18:23 AEST ]]> Airway Epithelial ITGB4 Deficiency Induces Airway Remodeling in a Mouse Model https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:50606 Wed 28 Feb 2024 15:56:56 AEDT ]]> Inhibition of γ-glutamyl transferase suppresses airway hyperresponsiveness and airway inflammation in a mouse model of steroid resistant asthma exacerbation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:52589 Wed 28 Feb 2024 15:29:42 AEDT ]]> Integrins are double-edged swords in pulmonary infectious diseases https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:52738 Wed 25 Oct 2023 08:40:27 AEDT ]]> miRNA-34b/c regulates mucus secretion in RSV-infected airway epithelial cells by targeting FGFR1 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:48903 Wed 19 Apr 2023 16:32:54 AEST ]]> miR-122 promotes virus-induced lung disease by targeting SOCS1 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:45152 Wed 15 Jan 2025 14:00:13 AEDT ]]> DNA methylation down-regulates integrin β4 expression in asthmatic airway epithelial cells https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:47164 Wed 14 Dec 2022 15:49:08 AEDT ]]> Antagonism of miR-328 increases the antimicrobial function of macrophages and neutrophils and rapid clearance of non-typeable Haemophilus Influenzae (NTHi) from infected lung https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:22005 haemophilus influenzae and pharmacological inhibition in mouse and human macrophages augments phagocytosis, the production of reactive oxygen species, and microbicidal activity. Moreover, inhibition of miR-328 in respiratory models of infection, steroid-induced immunosuppression, and smoke-induced emphysema enhances bacterial clearance. Thus, miRNA pathways can be targeted in the lung to enhance host defence against a clinically relevant microbial infection and offer a potential new anti-microbial approach for the treatment of respiratory diseases.]]> Wed 11 Apr 2018 16:14:13 AEST ]]> Expression profiling of differentiating eosinophils in bone marrow cultures predicts functional links between microRNAs and their target mRNAs https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:16812 Wed 11 Apr 2018 12:55:19 AEST ]]> Identification of the microRNA networks contributing to macrophage differentiation and function https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:28036 Wed 11 Apr 2018 12:47:04 AEST ]]> An alternate STAT6-independent pathway promotes eosinophil influx into blood during allergic airway inflammation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:15695 Wed 11 Apr 2018 12:28:11 AEST ]]> Bromodomain and extra terminal (BET) inhibitor suppresses macrophage-driven steroid-resistant exacerbations of airway hyper-responsiveness and inflammation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:30136 Wed 10 Nov 2021 15:14:29 AEDT ]]> Preventive effect of N-acetylcysteine in a mouse model of steroid resistant acute exacerbation of asthma https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:15991 Wed 02 Oct 2024 10:00:28 AEST ]]> Single-cell RNA transcriptomic analysis identifies Creb5 and CD11b-DCs as regulator of asthma exacerbations https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:50541 Wed 02 Aug 2023 13:55:11 AEST ]]> A selective a7 nicotinic acetylcholine receptor agonist, PNU-282987, attenuates ILC2s activation and alternaria-induced airway inflammation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:46307 Alternaria Alternata (AA)– induced airway inflammation. Methods: PNU-282987 was administered to mice that received recombinant IL-33 or AA intranasal challenges. Lung histological analysis and flow cytometry were performed to determine airway inflammation and the infiltration and activation of ILC2s. The previously published a7nAChR agonist GTS-21 was employed as a comparable reagent. ILC2s were isolated from murine lung tissue and cultured in vitro in the presence of IL-33, IL-2, and IL-7 with/without either PNU-282987 or GTS-21. The expression of the transcription factors GATA3, IKK, and NF-kB were also determined. Results: PNU-282987 and GTS-21 significantly reduced goblet cell hyperplasia in the airway, eosinophil infiltration, and ILC2s numbers in BALF, following IL-33 or AA challenge. In vitro IL-33 stimulation of isolated lung ILC2s showed a reduction of GATA3 and Ki67 in response to PNU-282987 or GTS-21 treatments. There was a significant reduction in IKK and NF-kB phosphorylation in the PNU-282987–treated group when compared to the GTS-21–treated ILC2s. Conclusion: PNU-282987 inhibits ILC2-associated airway inflammation, where its effects were comparable to that of GTS-21.]]> Tue 15 Nov 2022 10:22:01 AEDT ]]> Identification of MicroRNAs regulating the developmental pathways of bone marrow derived mast cells https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20638 Tue 14 Jan 2025 10:42:11 AEDT ]]> Activation of olfactory receptors on mouse pulmonary macrophages promotes monocyte chemotactic protein-1 production https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:15242 Thu 31 Oct 2024 12:13:56 AEDT ]]> ITGB4 deficiency induces mucus hypersecretion by upregulating MUC5AC in RSV-infected airway epithelial cells https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:45382 Thu 27 Oct 2022 16:12:30 AEDT ]]> Proteomic Analysis Reveals a Novel Therapeutic Strategy Using Fludarabine for Steroid-Resistant Asthma Exacerbation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:45231 Thu 27 Oct 2022 12:56:33 AEDT ]]> SULF1 expression is increased and promotes fibrosis through the TGF-β1/SMAD pathway in idiopathic pulmonary fibrosis https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:57213 Thu 27 Feb 2025 11:02:06 AEDT ]]> Single-cell transcriptomic analysis reveals the immune landscape of lung in steroid-resistant asthma exacerbation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:48011 + memory T cells were the predominant sources of interleukin (IL)-4 and IL-13 transcripts whose expressions were dexamethasone resistant. Production of IL-13 by these cells was validated by IL-13-reporter mice. Neutralization of IL-13 abolished HDM/LPS-induced airway hyperresponsiveness, airway inflammation, and decreased mucus hypersecretion. Furthermore, using Ingenuity Pathway Analysis systems, we identified canonical pathways and upstream regulators that regulate the activation of basophils, ILC2, and CD8+ memory T cells. Our study provides mechanistic insights and an important reference resource for further understanding of the immune landscape during asthma exacerbation.]]> Thu 23 Mar 2023 10:07:13 AEDT ]]> Potential Role of MicroRNAs in the Regulation of Antiviral Responses to influenza infection https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:43373 Thu 15 Sep 2022 15:46:34 AEST ]]> A critical role for the CXCL3/CXCL5/CXCR2 neutrophilic chemotactic axis in the regulation of type 2 responses in a model of rhinoviral-induced asthma exacerbation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:40298 Thu 11 May 2023 14:03:27 AEST ]]> Single-cell transcriptomic analysis reveals key immune cell phenotypes in the lungs of patients with asthma exacerbation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:46043 + T cells, and macrophages are significantly elevated in the bronchoalveolar lavage fluid of patients. A set of cytokines and intracellular transduction regulators are associated with asthma exacerbations and are shared across multiple cell clusters, forming a complicated molecular framework. An additional group of core exacerbation-associated modules is activated, including eukaryotic initiation factor 2 signaling, ephrin receptor signaling, and C-X-C chemokine receptor type 4 signaling in the subpopulations of CD8+ T cells (C1-a) and monocyte clusters (C7 clusters), which are associated with infection. Conclusion: Our study identified a significant number of severe asthma-associated genes that are differentially expressed by multiple cell clusters.]]> Thu 10 Nov 2022 14:51:33 AEDT ]]> Mouse models of severe asthma: understanding the mechanisms of steroid resistance, tissue remodelling and disease exacerbation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:33752 Thu 03 Feb 2022 12:18:24 AEDT ]]> MicroRNA-487b is a negative regulator of macrophage activation by targeting IL-33 production https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:30084 Thu 03 Feb 2022 12:18:07 AEDT ]]> Pathogenesis of steroid-resistant airway hyperresponsiveness: interaction between IFN-γ and TLR4/MyD88 pathways https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:7244 Sat 24 Mar 2018 08:33:46 AEDT ]]> Inhibition of arginase I activity by RNA interference attenuates IL-13-induced airways hyperresponsiveness https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:1061 Sat 24 Mar 2018 08:32:13 AEDT ]]> T helper-2 immunity regulates bronchial hyperresponsiveness in eosinophil-associated gastrointestinal disease in mice https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:2202 Sat 24 Mar 2018 08:30:10 AEDT ]]> Negative regulation of eosinophil recruitment to the lung by the chemokine monokine induced by INF-γ (Mig, CXCL9) https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:1493 Sat 24 Mar 2018 08:28:05 AEDT ]]> Emerging roles of pulmonary macrophages in driving the development of severe asthma https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:15983 50% of asthma-related healthcare costs. New investigations into the pathogenesis of glucocorticoid resistance in severe asthma indicate that pulmonary macrophages may play central roles in promoting airway inflammation, particularly in asthma that is resistant to steroid therapy. Importantly, factors that are linked to the activation of pulmonary macrophages may contribute to glucocorticoid resistance and severe asthma. Here, we review recent advances in understanding the roles of pulmonary macrophages in the mechanisms of glucocorticoid resistance and the pathogenesis of severe asthma. We discuss the role of macrophage phenotype, infection, IFN-γ, LPS, associated signaling pathways, TNF-α, MIF, and other macrophage-associated factors. Understanding the pathogenesis of steroid-resistant severe asthma will contribute to the identification of optimal therapeutic strategies for the effective management of the disease.]]> Sat 24 Mar 2018 08:23:36 AEDT ]]> Th2 cytokine antagonists: potential treatments for severe asthma https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:14610 Sat 24 Mar 2018 08:20:47 AEDT ]]> The emerging role of microRNAs in regulating immune and inflammatory responses in the lung https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:16016 Sat 24 Mar 2018 08:19:30 AEDT ]]> Potential therapeutic targets for steroid-resistant asthma https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:11176 Sat 24 Mar 2018 08:14:32 AEDT ]]> IL-27/IFN-γ induce MyD88-dependent steroid-resistant airway hyperresponsiveness by inhibiting glucocorticoid signaling in macrophages https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:11518 Sat 24 Mar 2018 08:10:22 AEDT ]]> Interleukin-13 regulates secretion of the tumor growth factor-β superfamily cytokine activin A in allergic airway inflammation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:11110 Sat 24 Mar 2018 08:10:16 AEDT ]]> Discovery, biology and therapeutic potential of RNA interference, microRNA and antagomirs https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:5035 Sat 24 Mar 2018 07:45:39 AEDT ]]> MicroRNA-9 regulates steroid-resistant airway hyperresponsiveness by reducing protein phosphatase 2A activity https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:22280 in silico and confirmed in luciferase reporter assays. miR-9 function was inhibited with sequence-specific antagomirs. The efficacy of DEX was assessed by quantifying glucocorticoid receptor (GR) cellular localization, protein phosphatase 2A (PP2A) activity, and AHR. Results: Exposure of pulmonary macrophages to IFN-γ/LPS synergistically induced miR-9 expression; reduced levels of its target transcript, protein phosphatase 2 regulatory subunit B (B56) δ isoform; attenuated PP2A activity; and inhibited DEX-induced GR nuclear translocation. Inhibition of miR-9 increased both PP2A activity and GR nuclear translocation in macrophages and restored steroid sensitivity in multiple models of steroid-resistant AHR. Pharmacologic activation of PP2A restored DEX efficacy and inhibited AHR. MiR-9 expression was increased in sputum of patients with neutrophilic but not those with eosinophilic asthma. Conclusion: MiR-9 regulates GR signaling and steroid-resistant AHR. Targeting miR-9 function might be a novel approach for the treatment of steroid-resistant asthma.]]> Sat 24 Mar 2018 07:17:42 AEDT ]]> Interferon-γ, pulmonary macrophages and airway responsiveness in asthma https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:22078 Sat 24 Mar 2018 07:15:16 AEDT ]]> TNF-α and macrophages are critical for respiratory syncytial virus-induced exacerbations in a mouse model of allergic airways disease https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:24758 Sat 24 Mar 2018 07:14:04 AEDT ]]> Employment of microRNA profiles and RNA interference and antagomirs for the characterization and treatment of respiratory disease https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:22118 Sat 24 Mar 2018 07:13:18 AEDT ]]> Airway epithelial-derived exosomes induce acute asthma exacerbation after respiratory syncytial virus infection https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:56270 Mon 26 Aug 2024 11:45:03 AEST ]]> Tuning photoresponse of graphene-black phosphorus heterostructure by electrostatic gating and photo-induced doping https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:44788 Mon 24 Oct 2022 09:17:58 AEDT ]]> ITGB4 is essential for containing HDM-induced airway inflammation and airway hyperresponsiveness https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:47479 Mon 23 Jan 2023 11:40:32 AEDT ]]> A new short-term mouse model of chronic obstructive pulmonary disease identifies a role for mast cell tryptase in pathogenesis https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:14158 Mon 13 Jan 2025 14:27:46 AEDT ]]> Airway epithelial integrin β4 suppresses allergic inflammation by decreasing CCL17 production https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:38495 Mon 09 May 2022 16:16:36 AEST ]]> Aberrant methylation of aging-related genes in asthma https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:40279 Mon 08 Aug 2022 11:55:28 AEST ]]> Airway epithelial overexpressed cathepsin K induces airway remodelling through epithelial–mesenchymal trophic unit activation in asthma https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:56531 Fri 25 Oct 2024 13:25:07 AEDT ]]> Innate lymphoid cells are double-edged swords under the mucosal barrier https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:48645 Fri 24 Mar 2023 13:44:36 AEDT ]]> Modeling T<inf>H</inf>2 responses and airway inflammation to understand fundamental mechanisms regulating the pathogenesis of asthma https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:33088 H2 cells), their associated cytokines, and eosinophils in the regulation of hallmark features of allergic asthma. Notably, we consider the complexity of type-2 responses and studies that have explored integrated signaling among classical TH2 cytokines (IL-4, IL-5, and IL-13), which together with CCL11 (eotaxin-1) regulate critical aspects of eosinophil recruitment, allergic inflammation, and airway hyper-responsiveness (AHR). Among our most important findings, we have provided evidence that the initiation of TH2 responses is regulated by airway epithelial cell-derived factors, including TRAIL and MID1, which promote TH2 cell development via STAT6-dependent pathways. Further, we highlight studies demonstrating that microRNAs are key regulators of allergic inflammation and potential targets for anti-inflammatory therapy. On the background of TH2 inflammation, we have demonstrated that innate immune cells (notably, airway macrophages) play essential roles in the generation of steroid-resistant inflammation and AHR secondary to allergen- and pathogen-induced exacerbations. Our work clearly indicates that understanding the diversity and spatiotemporal role of the inflammatory response and its interactions with resident airway cells is critical to advancing knowledge on asthma pathogenesis and the development of new therapeutic approaches.]]> Fri 24 Aug 2018 16:27:29 AEST ]]> Identification of IFN-γ and IL-27 as critical regulators of respiratory syncytial virus-induced exacerbation of allergic airways disease in a mouse model https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:33087 Fri 24 Aug 2018 16:25:53 AEST ]]> ITGB4 Deficiency in Airway Epithelium Aggravates RSV Infection and Increases HDM Sensitivity https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:52669 Fri 20 Oct 2023 09:24:41 AEDT ]]> ITGB4 deficiency in airway epithelia enhances HDM-induced airway inflammation through hyperactivation of TLR4 signaling pathway https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:52668 Fri 20 Oct 2023 09:24:13 AEDT ]]> ITGB4 deficiency induces DNA damage by downregulating HDAC1 in airway epithelial cells under stress stimulation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:52670 Fri 20 Oct 2023 09:23:42 AEDT ]]> Respiratory syncytial virus infection-induced mucus secretion by down-regulation of miR-34b/c-5p expression in airway epithelial cells https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:42298 Fri 19 Aug 2022 15:05:25 AEST ]]> Dysfunction of S100A4<sup>+</sup> effector memory CD8<sup>+</sup> T cells aggravates asthma https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:51730 Fri 15 Sep 2023 17:59:53 AEST ]]> GSTO1-1 is an upstream suppressor of M2 macrophage skewing and HIF-1α-induced eosinophilic airway inflammation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:40046 Fri 15 Jul 2022 10:05:42 AEST ]]> Lipopolysaccharide induces steroid-resistant exacerbations in a mouse model of allergic airway disease collectively through IL-13 and pulmonary macrophage activation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:41761 Fri 12 Aug 2022 11:42:33 AEST ]]> Aging in chronic lung disease: Will anti-aging therapy be the key to the cure? https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:56911 Fri 06 Dec 2024 16:47:11 AEDT ]]> IL-17A is a common and critical driver of impaired lung function and immunopathology induced by influenza virus, rhinovirus and respiratory syncytial virus https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:49776 Fri 02 Jun 2023 17:29:57 AEST ]]> Involvement of epithelia-derived exosomes in chronic respiratory diseases https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:49786 Fri 02 Jun 2023 17:27:41 AEST ]]>