https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 Isolates from colonic spirochetosis in humans show high genomic divergence and potential pathogenic features but are not detected using standard primers for the human microbiota https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:46669 Brachyspira aalborgi type strain, 513A. Also, 16S analysis of the mucosa-associated microbiota was performed in the cases and nonspirochetosis controls. We found one isolate to be of the species Brachyspira pilosicoli; all remaining isolates were of the species Brachyspira aalborgi. Besides displaying extensive genetic heterogeneity, the isolates harbored several mucin-degrading enzymes and other virulence-associated genes that could confer a pathogenic potential in the human colon. We also showed that 16S amplicon sequencing using standard primers for human microbiota studies failed to detect Brachyspira due to primer incompatibility. IMPORTANCE This is the first report of whole-genome analysis of clinical isolates from individuals with colonic spirochetosis. This characterization provides new opportunities in understanding the physiology and potentials of these bacteria that densely colonize the gut in the individuals infected. The observation that standard 16S amplicon primers fail to detect colonic spirochetosis may have major implications for studies searching for associations between members of the microbiota and clinical conditions such as irritable bowel syndrome (IBS) and should be taken into consideration in project design and interpretation of gastrointestinal tract microbiota in population-based and clinical settings.]]> Tue 29 Nov 2022 08:39:26 AEDT ]]> An increasing incidence of upper gastrointestinal disorders over 23 years: A prospective population-based study in Sweden https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:46068 Thu 10 Nov 2022 14:23:04 AEDT ]]> A randomly selected population sample undergoing colonoscopy: prevalence of the irritable bowel syndrome and the impact of selection factors https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:18602 Sat 24 Mar 2018 08:01:04 AEDT ]]> Colonoscopy findings in high-risk individuals compared to an average-risk control population https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:28377 Sat 24 Mar 2018 07:36:05 AEDT ]]> How individuals with the irritable bowel syndrome describe their own symptoms before formal diagnosis https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:26349 Sat 24 Mar 2018 07:35:54 AEDT ]]> Gastrointestinal recall questionnaires compare poorly with prospective patient diaries for gastrointestinal symptoms: Data from population and primary health centre samples https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:43800 Fri 30 Sep 2022 14:43:44 AEST ]]> Discriminant and convergent validity of the GSRS-IBS symptom severity measure for irritable bowel syndrome: a population study https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:39693 Fri 17 Jun 2022 16:07:04 AEST ]]> No distinct microbiome signature of irritable bowel syndrome found in a Swedish random population https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:46130 Fri 11 Nov 2022 15:21:48 AEDT ]]>