/manager/Index ${session.getAttribute("locale")} 5 Validation of an enzyme-linked immunoassay assay for osteocalcin, a marker of bone formation, in dried blood spots /manager/Repository/uon:46773 Wed 30 Nov 2022 13:14:41 AEDT ]]> Development and validation of an ELISA for a biomarker of thyroid dysfunction, thyroid peroxidase autoantibodies (TPO-Ab), in dried blood spots /manager/Repository/uon:39149 n = 12), we developed an indirect ELISA to measure TPO-Ab levels from one 3-mm DBS punch. The sensitivity and specificity of our assay for DBS samples ranged from 91.7–100% and 98.2–98.8%, respectively, using a cut-off value of ≥ 26 IU/mL. Intra-assay reliability for duplicate quality control DBS punches was 5.2%, while inter-assay reliability ranged from 11.5–24.4% for high, medium, and low DBS controls. Dilutional linearity ranged from 80 to 120%, and spike and recovery experiments indicated that the DBS matrix does not interfere with the detection of TPO-Ab. TPO-Ab levels remained stable in DBS samples stored at − 28 °C or − 80 °C, but decreased over time in DBS samples kept at 22 °C or at 37 °C. Conclusions: We developed an in-house, kit-independent indirect ELISA assay to determine individuals’ TPO-Ab positivity based on dried blood spots, representing a cost-effective method with potential applications in a range of research settings.]]> Thu 19 May 2022 15:40:39 AEST ]]> Physical function and activity among older adults in Jodhpur, India /manager/Repository/uon:25618 Thu 17 Feb 2022 09:25:42 AEDT ]]> Accelerometer-measured physical activity among older adults in urban India: Results of a study on global AGEing and adult health substudy /manager/Repository/uon:23069 Sat 24 Mar 2018 07:12:27 AEDT ]]> A dried blood spot-based method to measure levels of tartrate-resistant acid phosphatase 5b (TRACP-5b), a marker of bone resorption /manager/Repository/uon:45440 Fri 28 Oct 2022 12:58:16 AEDT ]]>