https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 Post-ejaculatory changes in the metabolic status of rat spermatozoa as measured by GC-MS https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:19948 Wed 27 Jul 2022 13:58:56 AEST ]]> Actin polymerisation during morphogenesis of the acrosome as spermatozoa undergo epididymal maturation in the tammar wallaby (Macropus eugenii) https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:1273 Wed 25 Sep 2024 16:00:24 AEST ]]> Lipid peroxidation in human spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27670 Wed 17 May 2017 14:00:30 AEST ]]> Gpx5 protects the family jewels https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:4655 Wed 16 Oct 2024 11:41:26 AEDT ]]> Accumulation of electrophilic aldehydes during postovulatory aging of mouse oocytes causes reduced fertility, oxidative stress, and apoptosis https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:24909 Wed 12 Aug 2020 09:08:42 AEST ]]> More than a simple lock and key mechanism: unraveling the intricacies of sperm-oocyte interactions https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:15653 Wed 11 Apr 2018 17:40:40 AEST ]]> Advantages of using the CRISPR/Cas9 system of genome editing to investigate male reproductive mechanisms using mouse models https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:24938 Wed 11 Apr 2018 16:25:07 AEST ]]> Apoptosis and DNA damage in human spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:23215 Wed 11 Apr 2018 15:05:01 AEST ]]> Significance of mitochondrial reactive oxygen species in the generation of oxidative stress in spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:4594 Wed 11 Apr 2018 14:21:05 AEST ]]> CRISPR/Cas9-mediated rapid generation of multiple mouse lines identified Ccdc63 as essential for spermiogenesis https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:26157 Wed 11 Apr 2018 13:31:01 AEST ]]> As the world grows: contraception in the 21st century https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:4511 Wed 11 Apr 2018 13:20:14 AEST ]]> Identification of the molecular chaperone, heat shock protein 1 (chaperonin 10), in the reproductive tract and in capacitating spermatozoa in the male mouse https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:4506 Wed 11 Apr 2018 10:11:11 AEST ]]> Mobile phone radiation induces reactive oxygen species production and DNA damage in human spermatozoa in vitro https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:7946 Wed 11 Apr 2018 10:00:06 AEST ]]> The role of molecular chaperones in spermatogenesis and the post-testicular maturation of mammalian spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:11890 Tue 24 Sep 2024 09:03:55 AEST ]]> The chaperonin containing TCP1 complex (CCT/TRiC) involved in mediating sperm-oocyte interaction https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:11888 Tue 24 Sep 2024 08:59:06 AEST ]]> Mass Spectrometry proteomics and the study of sperm cell biology https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:18188 Tue 23 Jun 2015 18:36:40 AEST ]]> Tyrosine phosphorylation activates surface chaperones facilitating sperm-zona recognition https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:2442 Tue 17 Sep 2024 10:09:02 AEST ]]> Electromagnetic radiation and oxidative stress in the male germ line https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:23922 Tue 01 Nov 2016 10:15:52 AEDT ]]> Analysis of the mechanism by which calcium negatively regulates the tyrosine phoshorylation cascade associated with sperm capacitation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:2527 Thu 19 Sep 2024 12:27:43 AEST ]]> The identification of mouse sperm-surface-associated proteins and characterization of their ability to act as decapacitation factors https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:1202 Thu 01 Aug 2019 17:27:56 AEST ]]> Proteomic insights into spermatozoa: critiques, comments and concerns https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:8340 Sat 24 Mar 2018 10:47:11 AEDT ]]> Biological and clinical significance of DNA damage in the male germ line https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:7489 Sat 24 Mar 2018 10:46:10 AEDT ]]> Proteomics of human spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:8464 Sat 24 Mar 2018 08:42:09 AEDT ]]> The spermostatic and microbicidal actions of quinones and maleimides: Toward a dual-purpose contraceptive agent https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:8305 Sat 24 Mar 2018 08:40:32 AEDT ]]> The biological significance of detergent-resistant membranes in spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:7212 Sat 24 Mar 2018 08:39:56 AEDT ]]> Global survey of protein expression during gonadal sex determination in mice https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:7528 Sat 24 Mar 2018 08:38:31 AEDT ]]> Composition and significance of detergent resistant membranes in mouse spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:7044 Sat 24 Mar 2018 08:37:51 AEDT ]]> DNA damage in human spermatozoa is highly correlated with the efficiency of chromatin remodeling and the formation of 8-hydroxy-2′-deoxyguanosine, a marker of oxidative stress https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:8206 Sat 24 Mar 2018 08:36:17 AEDT ]]> Phosphorylation and consequent stimulation of the tyrosine kinase c-Abl by PKA in mouse spermatozoa: its implications during capacitation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:7891 Sat 24 Mar 2018 08:35:08 AEDT ]]> Analysis of the relationships between oxidative stress, DNA damage and sperm vitality in a patient population: development of diagnostic criteria https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:9572 Sat 24 Mar 2018 08:34:47 AEDT ]]> Sperm DNA: organization, protection and vulnerability: from basic science to clinical applications: a position report https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:9576 Sat 24 Mar 2018 08:34:47 AEDT ]]> Ontogeny of tyrosine phosphorylation-signaling pathways during spermatogenesis and epididymal maturation in the mouse https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:3007 Sat 24 Mar 2018 08:33:06 AEDT ]]> Identification and characterization of a novel Mt-retrotransposon highly represented in the female mouse germline https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:1294 Sat 24 Mar 2018 08:32:44 AEDT ]]> Cis-unsaturated fatty acids stimulate reactive oxygen species generation and lipid peroxidation in human spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:1079 Sat 24 Mar 2018 08:32:08 AEDT ]]> Male reproductive health and the environment (editorial) https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:1032 Sat 24 Mar 2018 08:31:55 AEDT ]]> Contraceptive vaccines https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:1851 Sat 24 Mar 2018 08:31:16 AEDT ]]> The development of signal transduction pathways during epididymal maturation is calcium dependent https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:2526 Sat 24 Mar 2018 08:31:07 AEDT ]]> Localization and significance of molecular chaperones, heat shock protein 1, and tumor rejection antigen gp96 in the male reproductive tract and during capacitation and acrosome reaction https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:1595 Sat 24 Mar 2018 08:30:41 AEDT ]]> Tyrosine phosphorylation activates surface chaperones facilitating sperm-zona recognition https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:908 Sat 24 Mar 2018 08:30:00 AEDT ]]> CXCR4/SDF1 interaction inhibits the primordial to primary follicle transition in the neonatal mouse ovary https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:987 Sat 24 Mar 2018 08:29:49 AEDT ]]> Vitamin A regulation of BMP4 expression in the male germ line https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:1016 Sat 24 Mar 2018 08:29:47 AEDT ]]> Cyclic AMP-dependent tyrosine phosphorylation in tammar wallaby (Macropus eugenii) Spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:2392 Sat 24 Mar 2018 08:29:30 AEDT ]]> Definitive evidence for the nonmitochondrial production of superoxide anion by human spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:1163 Sat 24 Mar 2018 08:28:42 AEDT ]]> Seeds of concern https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:2302 Sat 24 Mar 2018 08:26:58 AEDT ]]> Identification and characterisation of a novel splice variant of mouse and rat cytochrome b5/cytochrome b5 reductase https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:2266 Sat 24 Mar 2018 08:26:55 AEDT ]]> Proteomic and functional analysis of human sperm detergent resistant membranes https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:13040 Sat 24 Mar 2018 08:16:38 AEDT ]]> Label-free quantitation of phosphopeptide changes during rat sperm capacitation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:9963 Sat 24 Mar 2018 08:14:26 AEDT ]]> Analysis of proteomic changes associated with sperm capacitation through the combined use of IPG-strip pre-fractionation followed by RP chromatography LC-MS/MS analysis https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:10935 Sat 24 Mar 2018 08:13:21 AEDT ]]> Prolactin exerts a prosurvival effect on human spermatozoa via mechanisms that involve the stimulation of Akt phosphorylation and suppression of caspase activation and capacitation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:11205 Sat 24 Mar 2018 08:12:44 AEDT ]]> New insights into sperm physiology and pathology https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:11318 Sat 24 Mar 2018 08:12:33 AEDT ]]> Sperm-zona pellucida interaction: molecular mechanisms and the potential for contraceptive intervention https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:11356 Sat 24 Mar 2018 08:11:57 AEDT ]]> Elucidation of the signaling pathways that underpin capacitation-associated surface phosphotyrosine expression in mouse spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:10106 Sat 24 Mar 2018 08:07:16 AEDT ]]> Paternal obesity, interventions, and mechanistic pathways to impaired health in offspring https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20202 Sat 24 Mar 2018 08:06:49 AEDT ]]> The role of sperm oxidative stress in male infertility and the significance of oral antioxidant therapy https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:17174 Sat 24 Mar 2018 08:06:30 AEDT ]]> Deficient tryptophan catabolism along the kynurenine pathway reveals that the epididymis is in a unique tolerogenic state https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:17478 −/−-deficient mice. In the caput epididymis of Ido1−/− animals, the lack of IDO activity was not compensated by other tryptophan-catabolizing enzymes and led to the loss of kynurenine production. The absence of IDO generated an inflammatory state in the caput epididymis as revealed by an increased accumulation of various inflammation markers. The absence of IDO also increased the tryptophan content of the caput epididymis and generated a parallel increase in caput epididymal protein content as a consequence of deficient proteasomal activity. Surprisingly, the lack of IDO expression had no noticeable impact on overall male fertility but did induce highly significant increases in both the number and the percentage of abnormal spermatozoa. These changes coincided with a significant decrease in white blood cell count in epididymal fluid compared with wild type mice. These data provide support for IDO playing a hitherto unsuspected role in sperm quality control in the epididymis involving the ubiquitination of defective spermatozoa and their subsequent removal.]]> Sat 24 Mar 2018 08:04:10 AEDT ]]> Use of titanium dioxide to find phosphopeptide and total protein changes during epididymal sperm maturation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:17528 Sat 24 Mar 2018 08:03:55 AEDT ]]> The John Hughes Memorial Lecture: aspects of sperm physiology-oxidative stress and the functionality of stallion spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20539 Sat 24 Mar 2018 08:02:43 AEDT ]]> Sperm capacitation: a distant landscape glimpsed but unexplored https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:17300 Sat 24 Mar 2018 08:01:46 AEDT ]]> The chaperonin containing TCP1 complex (CCT/TRiC) is involved in mediating sperm-oocyte interaction https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:17419 Sat 24 Mar 2018 08:01:38 AEDT ]]> Capacitation in the presence of methyl-β-cyclodextrin results in enhanced zona pellucida-binding ability of stallion spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:18685 Sat 24 Mar 2018 08:01:06 AEDT ]]> Melatonin prevents postovulatory oocyte aging in the mouse and extends the window for optimal fertilization in vitro https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20252 Sat 24 Mar 2018 07:59:55 AEDT ]]> Oxidative stress and ageing of the post-ovulatory oocyte https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:19945 Sat 24 Mar 2018 07:58:35 AEDT ]]> Causes and consequences of apoptosis in spermatozoa; contributions to infertility and impacts on development https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:19949 Sat 24 Mar 2018 07:58:32 AEDT ]]> The impact of sperm DNA damage in assisted conception and beyond: recent advances in diagnosis and treatment https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:19950 Sat 24 Mar 2018 07:58:32 AEDT ]]> Phosphoinositide 3-kinase signalling pathway involvement in a truncated apoptotic cascade associated with motility loss and oxidative DNA damage in human spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:17890 Sat 24 Mar 2018 07:56:25 AEDT ]]> Apoptosis in the germ line https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:17960 Sat 24 Mar 2018 07:56:21 AEDT ]]> Investigation of the mechanisms by which the molecular chaperone HSPA2 regulates the expression of sperm surface receptors involved in human spermoocyte recognition https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20323 Sat 24 Mar 2018 07:55:12 AEDT ]]> Age, the environment and our reproductive future: bonking baby boomers and the future of sex https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:21363 Sat 24 Mar 2018 07:51:26 AEDT ]]> Oxidative stress, spermatozoa and leukocytic infiltration: relationships forged by the opposing forces of microbial invasion and the search for perfection https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20031 Sat 24 Mar 2018 07:50:54 AEDT ]]> Head and flagella subcompartmental proteomic analysis of human spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20019 Sat 24 Mar 2018 07:50:53 AEDT ]]> The mouse sperm proteome characterized via IPG strip prefractionation and LC-MS/MS identification https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:5181 Sat 24 Mar 2018 07:47:49 AEDT ]]> The role of proteomics in understanding sperm cell biology https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:5193 Sat 24 Mar 2018 07:47:44 AEDT ]]> Need for standardization and confirmation of STS deletions on the Y chromosome (letter) https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:5470 Sat 24 Mar 2018 07:46:59 AEDT ]]> Just how safe is assisted reproductive technology for treating, male factor infertility? (editorial) https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:5508 Sat 24 Mar 2018 07:46:43 AEDT ]]> The rat sperm proteome characterized via IPG strip prefractionation and LC-MS/MS identification https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:5289 Sat 24 Mar 2018 07:46:30 AEDT ]]> Antioxidant systems and oxidative stress in the testes https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:6652 Sat 24 Mar 2018 07:46:20 AEDT ]]> Impact of estrogenic compounds on DNA integrity in human spermatozoa: evidence for cross-linking and redox cycling activities https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:5006 Sat 24 Mar 2018 07:44:14 AEDT ]]> Investigation of the role of SRC in capacitation-associated tyrosine phosphorylation of human spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:5003 Sat 24 Mar 2018 07:44:13 AEDT ]]> Glycogen synthase kinase 3 regulates acrosomal exocytosis in mouse spermatozoa via dynamin phosphorylation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27976 50 = 6.7 nM) led to a significant reduction in dynamin phosphorylation (10.3% vs. 27.3%; P < 0.001), acrosomal exocytosis (9.7% vs. 25.7%; P < 0.01), and in vitro fertilization (53% vs. 100%; P < 0.01). GSK3 was shown to be present in developing germ cells where it colocalized with dynamin in the peri-acrosomal domain. However, additional GSK3 was acquired by maturing mouse spermatozoa within the male reproductive tract, via a novel mechanism involving direct interaction of sperm heads with extracellular structures known as epididymal dense bodies. These data reveal a novel mode for the cellular acquisition of a protein kinase and identify a key role for GSK3 in the regulation of sperm maturation and acrosomal exocytosis.—Reid, A. T., Anderson, A. L., Roman, S. D., McLaughlin, E. A., McCluskey, A., Robinson, P. J., Aitken, R. J., Nixon, B. Glycogen synthase kinase 3 regulates acrosomal exocytosis in mouse spermatozoa via dynamin phosphorylation.]]> Sat 24 Mar 2018 07:38:43 AEDT ]]> Electrophoretic sperm isolation: optimization of electrophoresis conditions and impact on oxidative stress https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:25242 Sat 24 Mar 2018 07:38:18 AEDT ]]> Boronate probes for the detection of hydrogen peroxide release from human spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27394 Sat 24 Mar 2018 07:34:11 AEDT ]]> Characterization of an L-amino acid oxidase in equine spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27633 L-tryptophan > L-tyrosine) were substrates for this enzyme, eliciting the dose- and time-dependent generation of ROS via mechanisms that were enhanced by cell death. This unexpected result was confirmed by analyses of ROS generation in subcellular sperm fractions, which again located a majority of LAAO activity to the sperm head. Equine cryopreservation medium was shown to contain sufficient quantities of aromatic amino acids to activate the LAAO system and generate ROS. The biological significance of this activity was established in an experiment in which physiological concentrations of aromatic amino acids were found to suppress sperm motility but only if dead spermatozoa were present in the same suspension. The combination of aromatic amino acids and nonviable cells was also found to enhance the levels of lipid peroxidation in live spermatozoa. These results suggest the potential significance of LAAO activity in generating the oxidative stress associated with the cryopreservation of equine spermatozoa. It is possible that inhibitors of this enzyme system may facilitate the development of modified cryostorage regimes for clinical validation in vivo.]]> Sat 24 Mar 2018 07:34:05 AEDT ]]> Analysis of phosphopeptide changes as spermatozoa acquire functional competence in the epididymis demonstrates changes in the post-translational modification of Izumo1 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:25432 Sat 24 Mar 2018 07:31:54 AEDT ]]> Novel characterization of the HSPA2-stabilizing protein BAG6 in human spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27535 Sat 24 Mar 2018 07:28:58 AEDT ]]> Direct methods for the detection of reactive oxygen species in human semen samples https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27751 Sat 24 Mar 2018 07:27:46 AEDT ]]> Fertilization stimulates 8-hydroxy-2'-deoxyguanosine repair and antioxidant activity to prevent mutagenesis in the embryo https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:26581 Sat 24 Mar 2018 07:26:12 AEDT ]]> Investigation of the stallion sperm proteome by mass spectrometry https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27065 Sat 24 Mar 2018 07:25:20 AEDT ]]> Proteomic characterization of pig sperm anterior head plasma membrane reveals roles of acrosomal proteins in ZP3 binding https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27056 Sat 24 Mar 2018 07:25:20 AEDT ]]> Oxidative DNA damage in mouse sperm chromosomes: size matters https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:26315 Sat 24 Mar 2018 07:24:13 AEDT ]]> Reactive oxygen species: friend or foe https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:3878 Sat 24 Mar 2018 07:22:16 AEDT ]]> Bridging the gap between male and female fertility control: contraception-on-demand https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:4868 Sat 24 Mar 2018 07:18:43 AEDT ]]> Proteomic insights into the maturation and capacitation of mammalian spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:22257 Sat 24 Mar 2018 07:17:39 AEDT ]]> Dynamin regulates specific membrane fusion events necessary for acrosomal exocytosis in mouse spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:22263 in vitro induction of acrosomal exocytosis by progesterone, but not by the calcium ionophore A23187, and elicited a concomitant reduction of in vitro fertilization. In vivo treatment with these inhibitors also resulted in spermatozoa displaying reduced acrosome reaction potential. Dynamin 1 and 2 phosphorylation increased on progesterone treatment, and this was also selectively blocked by dynasore. On the basis of our collective data, we propose that dynamin could regulate specific membrane fusion events necessary for acrosomal exocytosis in mouse spermatozoa.]]> Sat 24 Mar 2018 07:17:38 AEDT ]]> Reactive oxygen species and sperm function-in sickness and in health https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:22254 Sat 24 Mar 2018 07:17:34 AEDT ]]> Electrophilic aldehydes generated by sperm metabolism activate mitochondrial reactive oxygen species generation and apoptosis by targeting succinate dehydrogenase https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:22207 R²= 0.89) in untreated populations of human spermatozoa emphasized the pathophysiological significance of these findings. The latter also provide a biochemical explanation for the self-perpetuating nature of oxidative stress in the male germ line, with the products of lipid peroxidation stimulating free radical generation by the sperm mitochondria in a positive feedback loop.]]> Sat 24 Mar 2018 07:11:16 AEDT ]]> Evidence for the involvement of PECAM-1 in a receptor mediated signal-transduction pathway regulating capacitation-associated tyrosine phosphorylation in human spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:1527 Mon 21 Oct 2024 13:30:16 AEDT ]]> RAB-Like 2 has an essential role in male fertility, sperm intra-flagellar transport, and tail assembly https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:15139 Mon 13 Jan 2025 14:14:31 AEDT ]]> The molecular chaperone HSPA2 plays a key role in regulating the expression of sperm surface receptors that mediate sperm-egg recognition https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:15122 Mon 13 Jan 2025 14:10:08 AEDT ]]> Identification of SRC as a key PKA-stimulated tyrosine kinase involved in the capacitation-associated hyperactivation of murine spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:1286 Fri 20 Sep 2024 10:19:29 AEST ]]> Identification of the insulin-like growth factor II receptor as a novel receptor for binding and invasion by Listeria monocytogenes https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:22119 Listeria monocytogenes causes a life-threatening disease known as listeriosis. The mechanism by which L. monocytogenes invades mammalian cells is not fully understood, but the processes involved may provide targets to prevent and treat listeriosis. Here, for the first time, we have identified the insulin-like growth factor II receptor (IGFIIR; also known as the cation-independent mannose 6-phosphate receptor CIM6PR or CD222) as a novel receptor for binding and invasion of Listeria species. Random peptide phage display was employed to select a peptide sequence by panning with immobilized L. monocytogenes cells; this peptide sequence corresponds to a sequence within the mannose 6-phosphate binding site of the IGFIIR. All Listeria spp. specifically bound the labeled peptide but not a control peptide, which was demonstrated using fluorescence spectrophotometry and fluorescence-activated cell sorting. Further evidence for binding of the receptor by L. monocytogenes and L. innocua was provided by affinity purification of the bovine IGFIIR from fetal calf serum by use of magnetic beads coated with cell preparations of Listeria spp. as affinity matrices. Adherence to and invasion of mammalian cells by L. monocytogenes was significantly inhibited by both the synthetic peptide and mannose 6-phosphate but not by appropriate controls. These observations indicate a role for the IGFIIR in the adherence and invasion of L. monocytogenes of mammalian cells, perhaps in combination with known mechanisms. Ligation of IGFIIR by L. monocytogenes may be a novel mechanism that contributes to the regulation of infectivity, possibly in combination with other mechanisms.]]> Fri 12 Feb 2016 15:22:05 AEDT ]]>