- Title
- Electrical methods for determining surface charge density and electrolyte composition at the lipid bilayer-solution interface
- Creator
- Laver, Derek
- Relation
- Advances in Planar Lipid Bilayers and Liposomes p. 87-105
- Publisher Link
- http://dx.doi.org/10.1016/S1554-4516(09)09004-8
- Publisher
- Elsevier
- Resource Type
- book chapter
- Date
- 2009
- Description
- In this chapter I will review electrical methods for inferring the surface potential and surface charge density on lipid bilayers and the electrolyte composition at the bilayer interface. I will review the early studies which measured electrostatic potentials between the bilayer interior and the external solution. These include methods based on ionophores, open circuit potential measurements, the inner field compensation and measurements of the ionic double-layer capacitance. I will then go on to review two methods which I developed for inferring changes in solute concentrations (solution transients) at a bilayer surface during solution changes which also allows simultaneous measurement of single channel activity. These methods (1) use fast solution changes with induce a shift in bilayer potential that can generate a transient, capacitive current through the bilayer under voltage-clamp conditions and (2) use ion channels embedded in the bilayer to sense solute concentrations during solution exchange.
- Subject
- surface potential; surface charge density; electrolytes; bilayer interface
- Identifier
- http://hdl.handle.net/1959.13/918018
- Identifier
- uon:8476
- Identifier
- ISBN:9780123748225
- Language
- eng
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