The CRY box: a second APCcdh¹-dependent degron in mammalian cdc20

Title: The CRY box: a second APCcdh¹-dependent degron in mammalian cdc20
Creator: Reis, Alexandra; Levasseur, Mark; Chang, Heng-Yu; Elliott, David J.; Jones, Keith T.
Relation: EMBO reports Vol. 7, Issue 10, p. 1040-1045
Publisher Link: http://dx.doi.org/10.1038/sj.embor.7400772
Publisher: Nature Publishing Group
Resource Type: journal article
Date: 2006
Description: Cdc20 and cdh1 are coactivators of the anaphase-promoting complex (APC). APCcdc²⁰ is necessary for the metaphase–anaphase transition and, at the end of mitosis, vertebrate cdc20 itself becomes a target for degradation through KEN-box-dependent APCcdh1 activity. By studying the degradation of fluorescent protein chimaeras in mammalian oocytes and early embryos, we found that cdc20 was degraded through two independent degradation signals (degrons), the KEN box and a newly described CRY box. In both oocytes and G1-stage embryos, the rate of degradation through the CRY box was greater than through the KEN box, although both were mediated by APCcdh¹. Thus, mammalian oocytes and embryos have the capacity to recognize two degrons in cdc20.
Subject: cell-cycle proteins; metaphase; meiosis; oocytes
Identifier: http://hdl.handle.net/1959.13/804218
Identifier: uon:6556
Identifier: ISSN:1469-221X
Language: eng
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