- Title
- EPAS1 expression contributes to maintenance of the primordial follicle pool in the mouse ovary
- Creator
- Martin, Jacinta H.; Bernstein, Ilana R.; Lyons, Jess M.; Brady, Ariel R.; Mabotuwana, Nishani S.; Stanger, Simone J.; De Oliveira, Camila Salum; Damyanova, Katerina B.; Nixon, Brett; Lord, Tessa
- Relation
- ARC.DE220100032 http://purl.org/au-research/grants/arc/DE220100032
- Relation
- Scientific Reports Vol. 14, Issue 1, no. 8770
- Publisher Link
- http://dx.doi.org/10.1038/s41598-024-59382-z
- Publisher
- Nature Publishing Group
- Resource Type
- journal article
- Date
- 2024
- Description
- Oxygen availability can have profound effects on cell fate decisions and survival, in part by regulating expression of hypoxia-inducible factors (HIFs). In the ovary, HIF expression has been characterised in granulosa cells, however, any requirement in oocytes remains relatively undefined. Here we developed a Hif2a/Epas1 germline-specific knockout mouse line in which females were fertile, however produced 40% fewer pups than controls. No defects in follicle development were detected, and quality of MII oocytes was normal, as per assessments of viability, intracellular reactive oxygen species, and spindle parameters. However, a significant diminishment of the primordial follicle pool was evident in cKO females that was attributed to accelerated follicle loss from postnatal day 6 onwards, potentially via disruption of the autophagy pathway. These data demonstrate the importance of HIF signalling in oocytes, particularly at the primordial follicle stage, and lend to the importance of controlling oxygen tension in the development of in vitro growth and maturation approaches for assisted reproduction.
- Subject
- oocyte; folliculogenesis; primordial follicle; hypoxia; HIF; EPAS1
- Identifier
- http://hdl.handle.net/1959.13/1502887
- Identifier
- uon:55287
- Identifier
- ISSN:2045-2322
- Rights
- © The Author(s) 2024. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4. 0/.
- Language
- eng
- Full Text
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