Brain tissue haemoglobin expression in saline-perfused vs non-perfused rodents

Walser, Marion; Karlsson, Lars; Motalleb, Reza; Isgaard, Jörgen; Kuhn, H. Georg; Åberg, N. David

Title: Brain tissue haemoglobin expression in saline-perfused vs non-perfused rodents
Creator: Walser, Marion; Karlsson, Lars; Motalleb, Reza; Isgaard, Jörgen; Kuhn, H. Georg; Åberg, N. David
Relation: Heliyon Vol. 10, Issue 1, no. e23343
Publisher Link: http://dx.doi.org/10.1016/j.heliyon.2023.e23343
Publisher: Elsevier
Resource Type: journal article
Date: 2024
Description: Haemoglobin beta (Hbb) and delta-aminolevulinate synthase 2 (Alas2) messenger RNA (mRNA) is mainly found in immature red blood cells, reticulocytes, and not in mature erythrocytes. However, these are also expressed in other tissues such as brain cells, mostly neurons. Therefore, exact quantification of neural tissue homogenates may be confounded by remaining blood in the brain vasculature that may give falsely high values of Hbb/Alas2 expression. To investigate and compare the contribution of local Hbb/Alas2 expression, we investigated mRNA expression locally in the hippocampus and prefrontal cortex, in post-sacrifice saline-perfused and non-perfused mice and rats. Although there was a higher level of Hbb/Alas2 transcripts in the non-perfused animals, there was a significant mRNA expression in perfused brains that could at most partially be explained by remaining blood. Finally, we suggest that saline-perfusion should be recommended for quantification of brain Hbb/Alas2 transcripts in homogenates.
Subject: brain; rodent; saline-perfused; non-perfused; haemoglobin beta; 5'-aminolevulinate synthase 2
Identifier: http://hdl.handle.net/1959.13/1500073
Identifier: uon:54844
Identifier: ISSN:2405-8440
Language: eng
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