- Title
- The Eastern Bandy Bandy Vermicella annulata, expresses high abundance of SVMP, CRiSP and Kunitz protein families in its venom proteome.
- Creator
- Tasoulis, Theo; Wang, C. Ruth; Sumner, Joanna; Dunstan, Nathan; Pukala, Tara L.; Isbister, Geoffrey K.
- Relation
- Journal of Proteomics Vol. 295, no. 105086
- Publisher Link
- http://dx.doi.org/10.1016/j.jprot.2024.105086
- Publisher
- Elsevier
- Resource Type
- journal article
- Date
- 2024
- Description
- The Australian elapid snake radiation (Hydrophiinae) has evolved in the absence of competition from other advanced snakes. This has resulted in ecological specialisation in Australian elapids and the potential for venom proteomes divergent to other elapids. We characterised the venom of the Australian elapid Vermicella annulata (eastern bandy bandy). The venom was analysed using a two-dimensional fractionation process consisting of reverse-phase high-performance liquid chromatography then sodium dodecyl sulphate polyacrylamide gel electrophoresis, followed by bottom-up proteomics. Resulting peptides were matched to a species-specific transcriptome and 87% of the venom was characterised. We identified 11 toxins in the venom from six families: snake venom metalloproteinases (SVMP; 24.2%; two toxins) that are class P-III SVMPs containing a disintegrin-like domain, three-finger toxins (3FTx; 21.6%; five toxins), kunitz peptides (KUN; 19.5%; one toxin), cysteine-rich secretory proteins (CRiSP; 18%; one toxin), and phospholipase A2 (PLA2; 4%; two toxins). The venom had low toxin diversity with five protein families having one or two toxins, except for 3FTx with five different toxins. V. annulata expresses an unusual venom proteome, with high abundances of CRiSP, KUN and SVMP, which are not normally highly expressed in elapid venoms. This unusual venom composition could be an adaptation to its specialised diet.
- Subject
- elapid; snake venom; Vermicella annulata; proteomics; venomics
- Identifier
- http://hdl.handle.net/1959.13/1499540
- Identifier
- uon:54722
- Identifier
- ISSN:1874-3919
- Language
- eng
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