- Title
- Transcriptomics identifies blunted immunomodulatory effects of vitamin D in people with multiple sclerosis
- Creator
- Yeh, Wei Z.; Lea, Rodney; Stankovich, Jim; Sampangi, Sandeep; Laverick, Louise; Van der Walt, Anneke; Jokubaitis, Vilija; Gresle, Melissa; Butzkueven, Helmut
- Relation
- Scientific Reports Vol. 14, no. 1436
- Publisher Link
- http://dx.doi.org/10.1038/s41598-024-51779-0
- Publisher
- Nature Publishing Group
- Resource Type
- journal article
- Date
- 2024
- Description
- Vitamin D deficiency is a risk factor for developing multiple sclerosis (MS). However, the immune effects of vitamin D in people with MS are not well understood. We analyzed transcriptomic datasets generated by RNA sequencing of immune cell subsets (CD4+, CD8+ T cells, B cells, monocytes) from 33 healthy controls and 33 untreated MS cases. We utilized a traditional bioinformatic pipeline and weighted gene co-expression network analysis (WGCNA) to determine genes and pathways correlated with endogenous vitamin D. In controls, CD4+ and CD8+ T cells had 1079 and 1188 genes, respectively, whose expressions were correlated with plasma 25-hydroxyvitamin D level (P < 0.05). Functional enrichment analysis identified association with TNF-alpha and MAPK signaling. In CD4+ T cells of controls, vitamin D level was associated with expression levels of several genes proximal to multiple sclerosis risk loci (P = 0.01). Genes differentially associated with endogenous vitamin D by case–control status were enriched in TNF-alpha signaling via NF-κB. WGCNA suggested a blunted response to vitamin D in cases relative to controls. Collectively, our findings provide further evidence for the immune effects of vitamin D, and demonstrate a differential immune response to vitamin D in cases relative to controls, highlighting a possible mechanism contributing to MS pathophysiology.
- Subject
- vitamin D; multiple sclerosis (MS); immune; genes
- Identifier
- http://hdl.handle.net/1959.13/1499487
- Identifier
- uon:54701
- Identifier
- ISSN:2045-2322
- Rights
- x
- Language
- eng
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