- Title
- TRAV26-2 T-Cell Receptor Expression is Associated with Mucosal Lymphocyte Response to Wheat Proteins in Patients with Functional Dyspepsia
- Creator
- Burns, Grace L.; Potter, Michael; Fairlie, Thomas; Cameron, Raquel; Bollipo, Steven; Irani, Mudar Zand; Foster, Robert; Gan, Lay T.; Shah, Ayesha; Koloski, Natasha; Foster, Pauls S.; Horvat, Jay C.; Mathe, Andrea; Walker, Marjorie M.; Powell, N; Veysey, Martin; Duncanson, Kerith; Holtmann, Gerald; Talley, Nicholas J.; Keely, Simon; Bruce, Jessica; Minahan, Kyra; Barnes, Jessica L.; Pryor, Jennifer; Nieva, Cheenie; Sherwin, Simonne; Cuskelly, Annalisa
- Relation
- Clinincal and Translational Gastroenterology Vol. 14, Issue 12, no. e00638
- Publisher Link
- http://dx.doi.org/10.14309/ctg.0000000000000638
- Publisher
- Wolters Kluwer Health
- Resource Type
- journal article
- Date
- 2023
- Description
- Introduction: An association between functional dyspepsia (FD) and wheat-containing foods has been reported in observational studies, however, an adaptive response has not been demonstrated. We examined whether antigens present in wheat could provoke a response from FD duodenal lymphocytes. Methods: Lamina propria mononuclear cells (LPMCs) were isolated from duodenal biopsies from 50 FD patients and 23 controls. LPMCs were exposed to gluten (0.2mg/mL) or gliadin (0.2mg/mL) for 24 hours. Flow cytometry was performed to phenotype lymphocytes. qPCR was used to measure expression of gliadin-associated T-cell receptor alpha variant (TRAV)26-2. Results: In response to gliadin (but not gluten) stimulation, the effector Th2-like population was increased in FD LPMCs compared to controls and unstimulated FD LPMCs. Duodenal gene expression of TRAV26-2 was decreased in FD compared to controls. We identified a positive association between gene expression of this T cell receptor variant and LPMC effector Th17-like cell populations in FD patients, but not controls following exposure to gluten, but not gliadin. Conclusion: Our findings suggest that gliadin exposure provokes a duodenal effector Th2-like response in FD patients, supporting the notion that food antigens drive responses in some patients. Further, these findings suggest that altered lymphocyte responses to wheat proteins play a role in FD pathogenesis.
- Subject
- functional dyspepsia; T cells; gluten sensitivity; Gliadin; immune response
- Identifier
- http://hdl.handle.net/1959.13/1496189
- Identifier
- uon:54158
- Identifier
- ISSN:2155-384X
- Rights
- x
- Language
- eng
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