- Title
- Allelic variations in high and low molecular weight glutenins at the Glu-Dt locus of Aegilops tauschii as a potential source for improving bread wheat quality
- Creator
- Rehman, A.; Evans, N.; Gianibelli, M. C.; Rose, R. J.
- Relation
- Australian Journal of Agricultural Research Vol. 59, Issue 5, p. 399-405
- Publisher Link
- http://dx.doi.org/10.1071/AR07229
- Publisher
- CSIRO Publishing
- Resource Type
- journal article
- Date
- 2008
- Description
- Aegilops tauschii, the donor of the D genome of hexaploid wheat, is accepted as a major contributor of disease resistance and bread-making quality attributes in cultivated wheat. High molecular weight (HMW) glutenins have a significant effect on the bread-making qualities of cultivated wheat. A large range of allelic variation in 424 Ae. tauschii accessions at the Glu-D1t locus for both x- and y-type glutenin subunits as well as at Glu-D3t was observed with SDS-PAGE using the total endosperm protein fraction. Only 4 accessions revealed more than 2 bands on SDS-PAGE. Seventeen new allelic combinations of both x- and y-type glutenin subunits at GluD-1t and 30 new allelic profiles at Glu-D3 were detected. These combinations comprise some lines with x- or y-type null forms. RP-HPLC analysis of accession Aus 18882, which showed 5 bands when the total endosperm protein fraction was resolved on SDS-PAGE, revealed 2 x-types and 1 y-type subunit banding pattern. RP-HPLC of the gliadin fraction exhibited an omega gliadin-like subunit. SDS-PAGE processing of the gliadin-free fraction of the same accession still exhibited the gliadin-like protein. An N-terminal protein sequence of the first 7 amino acids of the slowest moving novel x- type band of accession Aus18882 indicated its uniqueness, as no entries were found to contain this internal sequence. Demonstration of novel allelic combinations at Glu-D1 and Glu-D3 loci implies the potential for exploiting Ae. tauschii to increase the genetic variability of hexaploid wheat, particularly for bread-making qualities.
- Subject
- allelic variation; HMW and LMW glutenin subunits; novel combinations; bread wheat quality
- Identifier
- uon:5409
- Identifier
- http://hdl.handle.net/1959.13/43288
- Identifier
- ISSN:0004-9409
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