- Title
- Enabling peristalsis of human colon tumor organoids on microfluidic chips
- Creator
- Fang, Guocheng; Lu, Hongxu; Al-Nakashli, Russul; Chapman, Robert; Zhang, Yingqui; Ju, Lining Arnold; Lin, Gungun; Stenzel, Martina H.; Jin, Dayong
- Relation
- Biofabrication Vol. 14, Issue 1, no. 015006
- Publisher Link
- http://dx.doi.org/10.1088/1758-5090/ac2ef9
- Publisher
- Institute of Physics Publishing
- Resource Type
- journal article
- Date
- 2022
- Description
- Peristalsis in the digestive tract is crucial to maintain physiological functions. It remains challenging to mimic the peristaltic microenvironment in gastrointestinal organoid culture. Here, we present a method to model the peristalsis for human colon tumor organoids on a microfluidic chip. The chip contains hundreds of lateral microwells and a surrounding pressure channel. Human colon tumor organoids growing in the microwell were cyclically contracted by pressure channel, mimicking the in vivo mechano-stimulus by intestinal muscles. The chip allows the control of peristalsis amplitude and rhythm and the high throughput culture of organoids simultaneously. By applying 8% amplitude with 8 ∼ 10 times min−1, we observed the enhanced expression of Lgr5 and Ki67. Moreover, ellipticine-loaded polymeric micelles showed reduced uptake in the organoids under peristalsis and resulted in compromised anti-tumor efficacy. The results indicate the importance of mechanical stimuli mimicking the physiological environment when using in vitro models to evaluate nanoparticles. This work provides a method for attaining more reliable and representative organoids models in nanomedicine.
- Subject
- colon tumor organoids; peristalsis; microfluidic chip; micelle; nano drug carriers
- Identifier
- http://hdl.handle.net/1959.13/1475902
- Identifier
- uon:49688
- Identifier
- ISSN:1758-5082
- Language
- eng
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