- Title
- A novel role for milk fat globule-EGF factor 8 protein (MFGE8) in the mediation of mouse sperm–extracellular vesicle interactions
- Creator
- Trigg, Natalie A.; Stanger, Simone J.; Zhou, Wei; Skerrett-Byrne, David A.; Sipilä, Petra; Dun, Matthew D.; Eamens, Andrew L.; De Iuliis, Geoffry N.; Bromfield, Elizabeth G.; Roman, Shaun D.; Nixon, Brett
- Relation
- NHMRC.APP1147932 http://purl.org/au-research/grants/nhmrc/1147932 APP1138701 http://purl.org/au-research/grants/nhmrc/1138701
- Relation
- Proteomics Vol. 21, Issue 13-14, no. 2000079
- Publisher Link
- http://dx.doi.org/10.1002/pmic.202000079
- Publisher
- Wiley-VCH Verlag
- Resource Type
- journal article
- Date
- 2021
- Description
- Spermatozoa transition to functional maturity as they are conveyed through the epididymis, a highly specialized region of the male excurrent duct system. Owing to their transcriptionally and translationally inert state, this transformation into fertilization competent cells is driven by complex mechanisms of intercellular communication with the secretory epithelium that delineates the epididymal tubule. Chief among these mechanisms are the release of extracellular vesicles (EV), which have been implicated in the exchange of varied macromolecular cargo with spermatozoa. Here, we describe the optimization of a tractable cell culture model to study the mechanistic basis of sperm–extracellular vesicle interactions. In tandem with receptor inhibition strategies, our data demonstrate the importance of milk fat globule-EGF factor 8 (MFGE8) protein in mediating the efficient exchange of macromolecular EV cargo with mouse spermatozoa; with the MFGE8 integrin-binding Arg-Gly-Asp (RGD) tripeptide motif identified as being of particular importance. Specifically, complementary strategies involving MFGE8 RGD domain ablation, competitive RGD-peptide inhibition and antibody-masking of alpha V integrin receptors, all significantly inhibited the uptake and redistribution of EV-delivered proteins into immature mouse spermatozoa. These collective data implicate the MFGE8 ligand and its cognate integrin receptor in the mediation of the EV interactions that underpin sperm maturation.
- Subject
- epididymis; epididymosome; extracellular vesicle; milk fat globule-EGF factor 8 protein (MFGE8); sperm maturation; spermatozoa
- Identifier
- http://hdl.handle.net/1959.13/1474725
- Identifier
- uon:49349
- Identifier
- ISSN:1615-9853
- Rights
- Trigg, Natalie A.; Stanger, Simone J.; Zhou, Wei; Skerrett-Byrne, David A.; Sipilä, Petra; Dun, Matthew D.; Eamens, Andrew L.; De Iuliis, Geoffry N.; Bromfield, Elizabeth G.; Roman, Shaun D.; Nixon, Brett “A novel role for milk fat globule-EGF factor 8 protein (MFGE8) in the mediation of mouse sperm–-extracellular vesicle interactions”. Proteomics Vol. 21, Issue 13-14, no. 2000079 (2021), which has been published in final form at http://dx.doi.org/10.1002/pmic.202000079. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
- Language
- eng
- Full Text
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