- Title
- Molecular markers of type 2 airway inflammation are similar between eosinophilic severe asthma and eosinophilic chronic obstructive pulmonary disease
- Creator
- Fricker, Michael; McDonald, Vanessa M.; Winter, Natasha A.; Baines, Katherine J.; Wark, Peter A. B.; Simpson, Jodie L.; Gibson, Peter G.
- Relation
- NHMRC.1078579 http://purl.org/au-research/grants/nhmrc/1078579
- Relation
- Allergy Vol. 76, Issue 7, p. 2079-2089
- Publisher Link
- http://dx.doi.org/10.1111/all.14741
- Publisher
- Wiley-Blackwell
- Resource Type
- journal article
- Date
- 2021
- Description
- Background Airway and systemic eosinophilia are important treatable traits in both severe asthma and COPD. The molecular basis of eosinophilia in COPD is poorly understood but could involve type 2 cytokines (IL5, IL13) and prostaglandin D2 (PGD2). Methods This study included non-obstructive airways disease (OAD) controls (n = 19), a COPD cohort (n = 96) and a severe asthma cohort (n = 84). Demographics, exacerbation history, disease impact (SGRQ) and spirometry were assessed. Participants were categorized as eosinophilic using either sputum eosinophil proportion (≥3%) or blood eosinophil count (≥300/μL). Sputum type 2 inflammatory measures included PGD2 by ELISA and gene expression (qPCR) of IL5, IL13 and the haematopoietic PGD2 synthase (HPGDS). Results Type 2 markers did not differ across groups except HPGDS mRNA which was highest in non-OAD controls and lowest in COPD. IL5 and IL13 mRNA and PGD2 levels were significantly increased in eosinophilic vs non-eosinophilic severe asthma but did not differ between eosinophilic COPD and eosinophilic severe asthma or non-eosinophilic COPD. HPGDS expression was higher in eosinophilic severe asthma compared with eosinophilic COPD. Results were similar using sputum or blood eosinophil cut-offs. Sputum IL5 and IL13 were highly intercorrelated in severe asthma (r = 0.907, p < 0.001) and COPD (r = 0.824, p < 0.001), were moderately correlated with sputum eosinophils in severe asthma (IL5 r = 0.440, p < 0.001; IL13 r = 0.428, p < 0.001) and were weakly correlated in COPD (IL5 r = 0.245, p < 0.05; IL13 r = 0.317, p < 0.05). Conclusions Molecular markers of type 2 airway inflammation do not differ between eosinophilic asthma and eosinophilic COPD; however, the relationship between eosinophilia and type 2 airway markers appears weaker in COPD than in severe asthma.
- Subject
- biomarker; cytokine; eosinophil; obstructive airways disease; prostaglandin; sputum; SDG 3; Sustainable Development Goals
- Identifier
- http://hdl.handle.net/1959.13/1473799
- Identifier
- uon:49105
- Identifier
- ISSN:0105-4538
- Language
- eng
- Reviewed
- Hits: 746
- Visitors: 744
- Downloads: 0
| Thumbnail | File | Description | Size | Format |
|---|