- Title
- Expression of glucocorticoid receptor messenger ribonucleic acid transcripts in the human placenta at term
- Creator
- Johnson, Renée; Rennie, Natascha; Murphy, Vanessa; Zakar, Tamas; Clifton, Vicki; Smith, Roger
- Relation
- Journal of Clinical Endocrinology & Metabolism Vol. 93, Issue 12, p. 4887-4893
- Publisher Link
- http://dx.doi.org/10.1210/jc.2008-1077
- Publisher
- Endocrine Society
- Resource Type
- journal article
- Date
- 2008
- Description
- Context: Differential promoter use and alternative splicing generate a variety of glucocorticoid receptor (GR) mRNA transcripts, potentially altering the cortisol responsiveness of gestational tissues during pregnancy and labor. Objective: We examined GR mRNA transcript expression in term placentae before and after labor, in association with fetal sex and after glucocorticoid treatment. Design: RNA from 34 placentae and from eight placental explants incubated with glucocorticoids were analyzed for the GR mRNA variants GR-α, GR-β, GR-P, and GR-γ and the untranslated exon one variants 1A1, 1A2, 1A3, 1B, and 1C by quantitative RT-PCR. Main Outcome Measure: mRNA expression was assessed. Results: All GR mRNA variants examined were detected in the human placenta, with GR-α and GR-1C mRNA having the highest expression of GR splice variants and exon 1 variants, respectively. GR-P mRNA abundance decreased with spontaneous labor (P<0.01). GR-1A3 mRNA abundance changed with fetal sex, with a higher level in placentae of male fetuses (P <0.05). GR-1C was the preferential promoter for GR-α, GR-γ, and GR-P mRNA. GR-β mRNA was preferentially associated with GR-1A1. GR-P mRNA transcription switched to the GR-1A1 promoter after labor and to the GR-1A3 promoter in placentae from male fetuses. Glucocorticoid treatment significantly reduced transcription from promoters GR-1B and -1C and decreased GR-α and GR-P mRNA abundance. Conclusions: The human placenta expresses a variety of GR mRNA transcripts. GR-α mRNA transcribed from the 1C promoter generates the majority of placental GR. However, alterations in promoter use and alternative splicing may modulate responses to cortisol during stressful events.
- Subject
- glucocorticoid (GC) cortisol; human placenta; cortisol responsiveness; dexamethasone
- Identifier
- http://hdl.handle.net/1959.13/40372
- Identifier
- uon:4591
- Identifier
- ISSN:0021-972X
- Language
- eng
- Full Text
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