- Title
- Molecular Imprinting Using a Functional Chain Transfer Agent
- Creator
- Muang-Non, Phonlakrit; Lim, K. Fremielle; Katselas, Anthony; Holdsworth, Clovia I.
- Relation
- Molecules Vol. 27, Issue 4, no. 1162
- Publisher Link
- http://dx.doi.org/10.3390/molecules27041162
- Publisher
- MDPI AG
- Resource Type
- journal article
- Date
- 2022
- Description
- This study demonstrates the feasibility of molecular imprinting using a functional chain transfer agent sans a functional monomer. Ethylene glycol dimethacrylate (EGDMA)-based MIPs were synthesised in the presence of thioglycolic acid (TGA) possessing a carboxylic acid group, capable of interacting with the chosen test template R,S-(±)-propranolol (PNL) and a labile S-H bond to facilitate an efficient chain transfer reaction. Quantitative 1H NMR measurements showed high PNL and TGA incorporation within the MIP, indicating an efficient chain transfer process and a favourable interaction between PNL and TGA. TGA-50, with the lowest amount of CTA, showed the largest imprinting effect and an imprinting factor (IF) of 2.1. The addition of MAA to the formulation improved the binding capacity of PNL to the MIP but also increased NIP binding, resulting in a slightly decreased IF of 1.5. The Kd for the high-affinity sites of the TGA/MAA MIP were found to be two times lower (10 ± 1 µM) than that for the high-affinity sites of the TGA-only MIPs, suggesting that the incorporation of the functional monomer MAA increases the affinity towards the PNL template. Selectivity studies, cross-reactivity as well as binary competitive and displacement assays showed the TGA-based MIPs to be highly selective towards PNL against pindolol and slightly competitive against atenolol. The morphologies of the polymers were shown to be affected by the concentration of the TGA, transforming into discrete macrospheres (from small aggregates) at a higher TGA concentration.
- Subject
- MIP; molecular imprinting; thioglycolic acid; propranolol; chain transfer
- Identifier
- http://hdl.handle.net/1959.13/1457485
- Identifier
- uon:45351
- Identifier
- ISSN:1420-3049
- Rights
- This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. https://creativecommons.org/licenses/by/4.0/
- Language
- eng
- Full Text
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