- Title
- Obese asthmatics are characterized by altered adipose tissue macrophage activation
- Creator
- Periyalil, H. A.; Wood, L. G.; Wright, T. A.; Karihaloo, C.; Starkey, M. R.; Miu, A. S.; Baines, K. J.; Hansbro, P. M.; Gibson, P. G.
- Relation
- Clinical & Experimental Allergy Vol. 48, Issue 6, p. 641-649
- Publisher Link
- http://dx.doi.org/10.1111/cea.13109
- Publisher
- Wiley-Blackwell Publishing
- Resource Type
- journal article
- Date
- 2018
- Description
- Background: Adipose tissue-derived inflammation is linked to obesity-related comorbidities. This study aimed to quantify and immuno-phenotype adipose tissue macrophages (ATMs) from obese asthmatics and obese non-asthmatics and to examine associations between adipose tissue, systemic and airway inflammation. Methods: Visceral (VAT) adipose tissue and subcutaneous (SAT) adipose tissue were collected from obese adults undergoing bariatric surgery and processed to obtain the stromovascular fraction. Pro-inflammatory (M1) and anti-inflammatory (M2) macrophages were quantified by flow cytometry. Cytospins of induced sputum were stained for differential cell counts. Plasma C-reactive protein (CRP) and CD163 were measured by ELISA. Results: VAT contained a higher number of ATMs compared to SAT. A higher percentage of M1 ATMs was observed in VAT of obese asthmatics compared to obese non-asthmatics. The M1:M2 ratio in VAT was negatively associated with FEV₁%. Sputum macrophage count was correlated positively with M1 ATMs and negatively with M2 ATMs in VAT. In obese asthmatics, CRP was positively associated with M1:M2 ratio in VAT. There were no associations with CD163. An elevated ratio of M1:M2 ATMs was observed in VAT of obese asthmatics with increased disease severity. Conclusions and Clinical Relevance: Visceral inflammation with increased pro-inflammatory macrophages (M1) occurs in obese asthma and may be a determinant of systemic inflammation and asthma severity.
- Subject
- adipose tissue; asthma; inflammation; macrophage activation; obesity; SDG 3; Sustainable Development Goals
- Identifier
- http://hdl.handle.net/1959.13/1448849
- Identifier
- uon:43499
- Identifier
- ISSN:0954-7894
- Language
- eng
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