- Title
- Unique particle tracers for identifying chemical engineering products and processes
- Creator
- Neville, F.; Moreno-Atanasio, R.; Galvin, K. P.
- Relation
- Chemecha 2018. Chemeca 2018 (Queenstown, NZ 30 September - 3 October, 2018) p. 55.1-55.10
- Relation
- https://search.informit.org/doi/10.3316/informit.043383261905676
- Publisher
- Institution of Chemical Engineers
- Resource Type
- conference paper
- Date
- 2018
- Description
- Particle tracers are of great value to identify products and processes in a specific way. They can be used in a large number of applications including tracking of dangerous goods, anti-counterfeiting, assessment of purity of foodstuffs and in spillage control. In this work we aimed to produce particle tracers by synthesising silica particles labelled with unique DNA sequences and then identify the different particles through its attached DNA in a blind study. Two different DNA labels of 100 bases in length were designed artificially. This number corresponds to the maximum length that can be synthesised from individual bases and half of the usual minimum length required for base sequence identification. Therefore, we pushed the limits of the synthesis and identification techniques. Despite these limitations, the DNA could be attached to the particles and could be used directly for DNA amplification. Adsorption studies showed that the maximum binding efficiency of the DNA to the surface of the particles was approximately 90%. Furthermore, the binding could be modelled with a Langmuir isotherm and the maximum adsorption capacity was around 11 mg DNA/g particles. In addition, the DNA identification technique was able to distinguish the two different types of particles based on their DNA label sequences even though there were very small differences between them. This shows that the limits of the DNA synthesis and sequencing have been tested successfully and that the artificially designed DNA could be used as a unique particle tracer label.
- Subject
- particle tracing; DNA synthesis; DNA sequencing; synthesised silica
- Identifier
- http://hdl.handle.net/1959.13/1443591
- Identifier
- uon:42043
- Identifier
- ISBN:9781911446682
- Language
- eng
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