- Title
- Depolarization-associated CircRNA regulate neural gene expression and in some cases may function as templates for translation
- Creator
- Mahmoudi, Ebrahim; Kiltschewskij, Dylan; Fitzsimmons, Chantel; Cairns, Murray J.
- Relation
- NHMRC.1067137 http://purl.org/au-research/grants/nhmrc/1067137
- Relation
- Cells Vol. 9, Issue 1, no. 25
- Publisher Link
- http://dx.doi.org/10.3390/cells9010025
- Publisher
- MDPI
- Resource Type
- journal article
- Date
- 2020
- Description
- Circular RNAs (circRNAs) are a relatively new class of RNA transcript with high abundance in the mammalian brain. Here, we show that circRNAs expression in dierentiated neuroblastoma cells were significantly altered after depolarization with 107 upregulated and 47 downregulated circRNAs. This coincided with a global alteration in the expression of microRNA (miRNA) (n = 269)and mRNA (n = 1511) in depolarized cells, suggesting a regulatory axis of circRNA–miRNA–mRNA is involved in the cellular response to neural activity. In support of this, our in silico analysis revealed that the circular transcripts had the capacity to influence mRNA expression through interaction with common miRNAs. Loss-of-function of a highly expressed circRNA, circ-EXOC6B, resulted in altered expression of numerous mRNAs enriched in processes related to the EXOC6B function,suggesting that circRNAs may specifically regulate the genes acting in relation to their host genes. We also found that a subset of circRNAs, particularly in depolarized cells, were associated with ribosomes, suggesting they may be translated into protein. Overall, these data support a role for circRNAs in the modification of gene regulation associated with neuronal activity.
- Subject
- circular RNAs (circRNAs); circ-EXOC6B; depolarization; expression; translation
- Identifier
- http://hdl.handle.net/1959.13/1423928
- Identifier
- uon:37996
- Identifier
- ISSN:2073-4409
- Rights
- © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
- Language
- eng
- Full Text
- Reviewed
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