- Title
- Screening phytochemical content, antioxidant, antimicrobial and cytotoxic activities of Catharanthus roseus (L.) G. Don stem extract and its fractions
- Creator
- Pham, Hong Ngoc Thuy; Sakoff, Jennette A.; Vuong, Quan Van; Bowyer, Michael C.; Scarlett, Christopher J.
- Relation
- Biocatalysis and Agricultural Biotechnology Vol. 16, p. 405-411
- Publisher Link
- http://dx.doi.org/10.1016/j.bcab.2018.09.005
- Publisher
- Elsevier
- Resource Type
- journal article
- Date
- 2018
- Description
- The aims of this study were to screen the phytochemical content, antioxidant, antimicrobial and cytotoxic properties of the extract and its fractions prepared from Catharanthus roseus (L.) G. Don (C. roseus) stem. C. roseus stem was powdered and extracted with methanol using ultrasound-assisted extraction to obtain the crude extract. The crude extract was further fractioned using liquid-liquid extraction technique to obtain extracts of increasing polarity including n-butanol and residual aqueous fractions. The crude extract and its derived fractions were then subjected to phytochemical screening and assayed for antioxidant, antimicrobial and cytotoxic activities. Results showed that the n-butanol fraction contained the highest levels of saponins and phenolics (3037.54 mg ESE/g and 77.87 mg GAE/g, respectively) and possessed the strongest antioxidant capacity amongst the tested extracts. HPLC analysis revealed that this n-butanol fraction had high levels of apigenin and kaempferol, whereas the aqueous fraction contained a high level of gallic acid. The n-butanol fraction was found to effectively inhibit the activity of Escherichia coli and Staphylococccus lugdunensis. The n-butanol fraction also possessed strong cytotoxic activity in vitro against a wide range of cancer cell lines including A2780 (ovarian), H460 (lung), A431 (skin), MIA PaCa-2 (pancreas), Du145 (prostate), HT29 (colon), MCF-7 (breast), BE2-C (neuroblastoma), SJ-G2, U87 and SMA (glioblastoma) at low doses (GI50 values of 5.2−21.0 µg/mL). These results indicate that the n-butanol fraction prepared from C. roseus stem is a rich source of bioactive compounds which can be isolated for further evaluation as potential antimicrobial drugs or antitumor therapeutic agents.
- Subject
- Catharanthus roseus; phytochemical; antioxidant; antimicrobial; cytotoxic activities
- Identifier
- http://hdl.handle.net/1959.13/1412941
- Identifier
- uon:36554
- Identifier
- ISSN:1878-8181
- Language
- eng
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