- Title
- Establishment of coral-bacteria symbioses reveal changes in the core bacterial community with host ontogeny
- Creator
- Bernasconi, Rachele; Stat, Michael; Koenders, Annette; Paparini, Andrea; Bunce, Michael; Huggett, Megan J.
- Relation
- Frontiers in Microbiology Vol. 10, no. 1529
- Publisher Link
- http://dx.doi.org/10.3389/fmicb.2019.01529
- Publisher
- Frontiers Research Foundation
- Resource Type
- journal article
- Date
- 2019
- Description
- Bacterial communities are fundamental symbionts of corals. However, the process by which bacterial communities are acquired across the life history of corals, particularly in larval and early juvenile stages, is still poorly characterized. Here, transfer of bacteria of the Scleractinian coral Acropora digitifera from adults to spawned egg-sperm bundles was analyzed, as well as acquisition across early developmental stages (larvae and newly settled spat), and 6-month-old juveniles. Larvae were reared under manipulated environmental conditions to determine the source (maternal, seawater, or sediment) of bacteria likely to establish symbiotic relationships with the host using amplicon sequencing of the 16S rRNA gene. Maternal colonies directly transferred bacteria from the families Rhodobacteraceae, Cryomorphaceae, and Endozoicimonaceae to egg-sperm bundles. Furthermore, significant differences in the microbial community structure were identified across generations, yet the structure of the coral bacterial community across early life history stages was not impacted by different environmental rearing conditions. These data indicate that the uptake and structure of bacterial communities is developmentally, rather than environmentally, regulated. Both maternal coral colonies and ubiquitous bacteria found across environmental substrates represent a potential source of symbionts important in establishing the coral microbiome. Uniquely, we report the presence of variation with ontogeny of both the core and resident bacterial communities, supporting the hypothesis that microbial communities are likely to play specific roles within the distinct life history stages of the coral host.
- Subject
- core bacterial communities; symbiosis; 16S rRNA; metabarcoding; bacterial communities; seawater; sediment; transmission
- Identifier
- http://hdl.handle.net/1959.13/1405316
- Identifier
- uon:35467
- Identifier
- ISSN:1664-302X
- Rights
- © 2019 Bernasconi, Stat, Koenders, Paparini, Bunce and Huggett. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
- Language
- eng
- Full Text
- Reviewed
- Hits: 10838
- Visitors: 7940
- Downloads: 226
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | ATTACHMENT02 | Publisher version (open access) | 7 MB | Adobe Acrobat PDF | View Details Download |