- Title
- Extracellular vesicles with altered tetraspanin CD9 and CD151 levels confer increased prostate cell motility and invasion
- Creator
- Brzozowski, Joshua S.; Bond, Danielle R.; Skelding, Kathryn A.; Weidenhofer, Judith; Jankowski, Helen; Goldie, Belinda J.; Burchell, Rachel; Naudin, Crystal; Smith, Nathan D.; Scarlett, Christopher J.; Larsen, Martin R.; Dun, Matthew D.
- Relation
- Scientific Reports Vol. 8, no. 8822
- Publisher Link
- http://dx.doi.org/10.1038/s41598-018-27180-z
- Publisher
- Nature Publishing Group
- Resource Type
- journal article
- Date
- 2018
- Description
- To facilitate intercellular communication, cells release nano-sized, extracellular vesicles (EVs) to transfer biological cargo to both local and distant sites. EVs are enriched in tetraspanins, two of which (CD9 and CD151) have altered expression patterns in many solid tumours, including prostate cancer, as they advance toward metastasis. We aimed to determine whether EVs from prostate cells with altered CD9 and CD151 expression could influence cellular behaviour and increase the metastatic capabilities of non-tumourigenic prostate cells. EVs were isolated by ultrafiltration and characterised for their tetraspanin expression and size distribution. iTRAQ was used to identify differences between RWPE1 and tetraspanin-modified RWPE1 EV proteomes, showing an enrichment in protein degradation pathways. Addition of EVs from RWPE1 cells with reduced CD9 or increased CD151 abundance resulted in increased invasion of RWPE1 cells, and increased migration in the case of high CD151 abundance. We have been able to show that alteration of CD9 and CD151 on prostate cells alters the proteome of their resultant EVs, and that these EVs can enhance the migratory and invasive capabilities of a non-tumourigenic prostate cellular population. This work suggests that cellular tetraspanin levels can alter EVs, potentially acting as a driver of metastasis in prostate cancer.
- Subject
- intercellular communication; extracellular vesicles; tetraspanins; CD9; CD151; prostate cancer; prostate cells
- Identifier
- http://hdl.handle.net/1959.13/1392945
- Identifier
- uon:33473
- Identifier
- ISSN:2045-2322
- Rights
- © The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
- Language
- eng
- Full Text
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