- Title
- Cytokine responses to two common respiratory pathogens in children are dependent on interleukin-1β
- Creator
- Chen, Alice C-H; Xi, Yang; Masters, Ian B.; Buntain, Helen M.; Chang, Anne B.; Upham, John W.; Carroll, Melanie; Petsky, Helen L.; Gardiner, Samantha J.; Pizzutto, Susan J.; Yerkovich, Stephanie T.; Baines, Katherine J.; Gibson, Peter G.; Hodge, Sandra
- Relation
- Funding BodyNHMRCGrant Number1042601 http://purl.org/au-research/grants/nhmrc/1042601
- Relation
- ERS Monograph Vol. 3, Issue 4, no. 00025-2017
- Publisher Link
- http://dx.doi.org/10.1183/23120541.00025-2017
- Publisher
- European Respiratory Society
- Resource Type
- journal article
- Date
- 2017
- Description
- Protracted bacterial bronchitis (PBB) in young children is a common cause of prolonged wet cough and may be a precursor to bronchiectasis in some children. Although PBB and bronchiectasis are both characterised by neutrophilic airway inflammation and a prominent interleukin (IL)-1β signature, the contribution of the IL-1β pathway to host defence is not clear. This study aimed to compare systemic immune responses against common pathogens in children with PBB, bronchiectasis and control children and to determine the importance of the IL-1β pathway. Non-typeable Haemophilus influenzae (NTHi) stimulation of peripheral blood mononuclear cells (PBMCs) from control subjects (n=20), those with recurrent PBB (n=20) and bronchiectasis (n=20) induced high concentrations of IL-1β, IL-6, interferon (IFN)-γ and IL-10. Blocking with an IL-1 receptor antagonist (IL-1Ra) modified the cellular response to pathogens, inhibiting cytokine synthesis by NTHistimulated PBMCs and rhinovirus-stimulated PBMCs (in a separate PBB cohort). Inhibition of IFN-γ production by IL-1Ra was observed across multiple cell types, including CD3⁺ T cells and CD56⁺ NK cells. Our findings highlight the extent to which IL-1β regulates the cellular immune response against two common respiratory pathogens. While blocking the IL-1β pathway has the potential to reduce inflammation, this may come at the cost of protective immunity against NTHi and rhinovirus.
- Subject
- immune responses; children; protracted bacterial bronchitis; bronchiectasis; PBB
- Identifier
- http://hdl.handle.net/1959.13/1387715
- Identifier
- uon:32658
- Identifier
- ISSN:2312-508X
- Rights
- Copyright ©ERS 2017. This article is open access and distributed under the terms of the Creative Commons Attribution Non-Commercial Licence 4.0.
- Language
- eng
- Full Text
- Reviewed
- Hits: 8878
- Visitors: 9211
- Downloads: 399
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | ATTACHMENT02 | Publisher version (open access) | 721 KB | Adobe Acrobat PDF | View Details Download |