- Title
- MicroRNA-645 is an oncogenic regulator in colon cancer
- Creator
- Guo, S. T.; Guo, X. Y.; Jin, L.; Zhang, X. D.; Jiang, C. C.; Wang, J.; Wang, C. Y.; Yang, R. H.; Wang, F. H.; Li, X. Y.; Hondermarck, H.; Thorne, R. F.; Wang, Y. F.
- Relation
- NHMRC.1026458 http://purl.org/au-research/grants/nhmrc/1026458
- Relation
- Oncogenesis Vol. 6, no. e335
- Publisher Link
- http://dx.doi.org/10.1038/oncsis.2017.37
- Publisher
- Nature Publishing Group
- Resource Type
- journal article
- Date
- 2017
- Description
- Despite advances in early diagnosis and the development of molecularly targeted therapy, curative treatment of colon cancer once it has metastasized is yet to be accomplished. This is closely associated with deregulated CRC cell proliferation and resistance to apoptosis. Here we reveal that upregulation of microRNA-645 (miR-645) through DNA copy number gain is responsible for enhanced proliferation and resistance to apoptosis in colon cancer. MiR-645 was upregulated in most colon cancer tissues related to adjacent normal mucosa. This appeared to be associated with amplification of a section of chromosome 20q13.13, where miR-645 is located. Inhibition of miR-645 reduced proliferation and enhanced sensitivity to apoptosis triggered by the chemotherapeutic drugs 5-fluorouracil and cisplatin in CRC cells, and retarded colon cancer xenograft growth. Conversely, overexpression of miR-645 in normal colon epithelial cells enhanced proliferation and triggered anchorage-independent cell growth. Although SRY-related HMG-box 30 (SOX30) was identified as a miR-645 target, its expression was only partially affected by miR-645, suggesting that miR-645 is a fine-tuning mechanism of SOX30 expression. Moreover, overexpression of SOX30 only moderately inhibited promotion of CRC cell proliferation by miR-645, indicating that miR-645 may have more targets that contribute to its pro-proliferation effect in colon cancer. Together, this study reveals that miR-645 can regulate oncogenesis in colon cancer with SOX30 being one of its targets
- Subject
- gastrointestinal cancer; oncogenes; colon cancer; cancer
- Identifier
- http://hdl.handle.net/1959.13/1351652
- Identifier
- uon:30753
- Identifier
- ISSN:2157-9024
- Rights
- Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
- Language
- eng
- Full Text
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