- Title
- Preparation and binding evaluation of histamine-imprinted microspheres via conventional thermal and RAFT-mediated free-radical polymerization
- Creator
- Romano, Edwin F.; So, Regina C.; Donne, Scott W.; Holdsworth, Clovia I.
- Relation
- ACS Omega Vol. 1, Issue 4, p. 518-531
- Publisher Link
- http://dx.doi.org/10.1021/acsomega.6b00144
- Publisher
- American Chemical Society
- Resource Type
- journal article
- Date
- 2016
- Description
- Elevated histamine (HTM) levels are closely linked to food poisoning as well as to pathophysiological allergic diseases. In this study, HTM-imprinted, solution-processable microspheres were prepared via high-dilution conventional thermal polymerization (CTP) and controlled radical polymerization (CRP) using ethylene glycol dimethacrylate (80 or 90 wt %) and methacrylic acid at 60 °C in acetonitrile and evaluated as recognition materials for sensing applications. The polymers were selective to HTM in binding studies, cross-rebinding, and competitive binding assays against the HTM analogues histidine, imidazole, and tryptamine. The selective binding capacity was significantly higher with CTP-80 (on the basis of mass: 21.0 μmol/g and surface area: 8.08 × 10-2 μmol/m2) than that with both CTP-90 (8.47 μmol/g, 4.49 × 10–2 μmol/m2) and CRP-80 (9.00 μmol/g, 1.19 × 10–2 μmol/m2).
- Subject
- biosensors; distribution function; ionic strength; mechanical properties; molecular association; molecular structure-property relationship; polymerization (polymers); polymers; porosity
- Identifier
- http://hdl.handle.net/1959.13/1346145
- Identifier
- uon:29793
- Identifier
- ISSN:2470-1343
- Rights
- This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
- Language
- eng
- Full Text
- Reviewed
- Hits: 1655
- Visitors: 1932
- Downloads: 344
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | ATTACHMENT02 | Publisher version (open access) | 2 MB | Adobe Acrobat PDF | View Details Download |