Imaging fluorescent protein fusions in live bacteria

Title: Imaging fluorescent protein fusions in live bacteria
Creator: Doherty, Geoff; Mettrick, Karla; Grainge, Ian; Lewis, Peter J.
Relation: Systems Biology of Bacteria p. 107-126
Relation: Methods in Microbiology 39
Publisher Link: http://dx.doi.org/10.1016/B978-0-08-099387-4.00004-1
Publisher: Academic Press
Resource Type: book chapter
Date: 2012
Description: The use of fluorescent protein fusions has revolutionised our understanding of bacterial cell structure. Here we present a brief overview of the molecular toolkits available for the production of functional fluorescent protein fusions in Gram positive and Gram negative organisms. We highlight considerations for performing live cell imaging including equipment requirements and simple approaches to image processing.
Subject: green fluorescent protein; protein fusions; live cell imaging; fluorescence microscopy
Identifier: http://hdl.handle.net/1959.13/1328778
Identifier: uon:25993
Identifier: ISBN:9780080993874
Language: eng