- Title
- Kinetics of PAH degradation by a new acid-metal-tolerant Trabulsiella isolated from the MGP site soil and identification of its potential to fix nitrogen and solubilize phosphorous
- Creator
- Kuppusamy, Saranya; Thavamani, Palanisami; Megharaj, Mallavarapu; Lee, Yong Bok; Naidu, Ravi
- Relation
- Journal of Hazardous Materials Vol. 307, p. 99-107
- Publisher Link
- http://dx.doi.org/10.1016/j.jhazmat.2015.12.068
- Publisher
- Elsevier
- Resource Type
- journal article
- Date
- 2016
- Description
- Development of an efficient bioinoculum is considered as an appropriate remedial approach to treat the PAHs-metal mixed contaminated sites. Therefore, we aimed to isolate a degrader able to exert an outstanding PAH catabolic potential with added traits of pH-metal-resistance, N-fix or P-solubilization from a manufactured gas plant site soil. The identified strain (MTS-6) was a first low and high molecular weight (LMW and HMW) PAHs degrading Trabulsiella sp. tolerant to pH 5. MTS-6 completely degraded the model 3 [150 mg L−1 phenanthrene (Phe)], 4 [150 mg L−1 pyrene (Pyr)] and 5 [50 mg L−1 benzo[a]pyrene (BaP)] ring PAHs in 6, 25 and 90 days, respectively. Presence of co-substrate (100 mg L−1 Phe) increased the biodegradation rate constant (k) and decreased the half-life time (t1/2) of HMW PAHs (100 mg L−1 Pyr or 50 mg L−1 BaP). The strain fixed 47 μg mL−1 N and solubilized 58 μg mL−1 P during PAH metabolism and exhibited an EC50 value of 3–4 mg L−1 for Cu, Cd, Pb and Zn. Over 6 mg L−1 metal levels was lethal for the microbe. The identified bacterium (MTS-6) with exceptional multi-functional traits opens the way for its exploitation in the bioremediation of manufactured gas plant sites in a sustainable way by employing bioaugmentation strategy.
- Subject
- mixed contamination; HMW PAHs degradation; diazotrophic bacteria; phosphate solubilizer; heavy metal resistance
- Identifier
- http://hdl.handle.net/1959.13/1321603
- Identifier
- uon:24409
- Identifier
- ISSN:0304-3894
- Language
- eng
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