- Title
- Bioremediation potential of a highly mercury resistant bacterial strain Sphingobium SA2 isolated from contaminated soil
- Creator
- Mahbub, Khandaker Rayhan; Krishnan, Kannan; Megharaj, Mallavarapu; Naidu, Ravi
- Relation
- Chemosphere Vol. 144, Issue February 2016, p. 330-337
- Publisher Link
- http://dx.doi.org/10.1016/j.chemosphere.2015.08.061
- Publisher
- Elsevier
- Resource Type
- journal article
- Date
- 2016
- Description
- A mercury resistant bacterial strain, SA2, was isolated from soil contaminated with mercury. The 16S rRNA gene sequence of this isolate showed 99% sequence similarity to the genera Sphingobium and Sphingomonas of α-proteobacteria group. However, the isolate formed a distinct phyletic line with the genus Sphingobium suggesting the strain belongs to Sphingobium sp. Toxicity studies indicated resistance to high levels of mercury with estimated EC₅₀ values 4.5 mg L-1 and 44.15 mg L-1 and MIC values 5.1 mg L-1 and 48.48 mg L-1 in minimal and rich media, respectively. The strain SA2 was able to volatilize mercury by producing mercuric reductase enzyme which makes it potential candidate for remediating mercury. ICP-QQQ-MS analysis of Hg supplemented culture solutions confirmed that almost 79% mercury in the culture suspension was volatilized in 6 h. A very small amount of mercury was observed to accumulate in cell pellets which was also evident according to ESEM-EDX analysis. The mercuric reductase gene merA was amplified and sequenced. The deduced amino acid sequence demonstrated sequence homology with α-proteobacteria and Ascomycota group.
- Subject
- mercury; Hg resistance; Hg volatilization; Hg removal; <i>Sphingobium</i>
- Identifier
- http://hdl.handle.net/1959.13/1319929
- Identifier
- uon:24015
- Identifier
- ISSN:0045-6535
- Language
- eng
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