- Title
- The control of prostaglandin endoperoxide H-synthase-2 expression in the human chorion laeve at term
- Creator
- Johnson, Renee F.; Mitchell, Carolyn M.; Giles, Warwick B.; Walters, William A.; Zakar, Tamas
- Relation
- Journal Of The Society For Gynecologic Investigation Vol. 10, Issue 4, p. 222-230
- Relation
- http://rsx.sagepub.com/cgi/content/abstract/10/4/222
- Publisher
- Elsevier Science Inc
- Resource Type
- journal article
- Date
- 2003
- Description
- Objective: Prostaglandin endoperoxide H synthase-2 (PGHS-2), the key enzyme of prostaglandin biosynthesis in gestational tissues, is expressed in the chorion laeve at term. We have determined the mechanisms that control the level of PGHS-2 mRNA in the chorion membrane in order to assess the significance of chorion-derived prostaglandins in term labor. Methods: Chorion membranes were collected after elective cesarean delivery (CD, n = 21) and after spontaneous labor (SL, n = 20) at term. The PGHS-2 gene transcription rate was measured by transcriptional run-on, and PGHS-2 mRNA and heterogeneous RNA (hnRNA) abundance was determined by quantitative real-time reverse transcriptase polymerase chain reaction. PGHS-2 mRNA stability, PGHS-2 hnRNA processing rate, and the short-term dynamics of the two RNA species were characterized in 0-24-hour-long tissue incubations. Results: The transcriptional activity of the PGHS-2 gene predicted (P < .02) the abundance of PGHS-2 mRNA and hnRNA in individual tissues. PGHS-2 gene activity and hnRNA processing rate were not different in the CD and SL groups. PGHS-2 mRNA was constitutively stable before and after labor, and its abundance spontaneously increased sixfold in tissues incubated for 24 hours. At the same time, PGHS-2 gene activity decreased by 80% within 2 hours and rebounded to 60% of its initial level by 24 hours. Conclusion: PGHS-2 mRNA is highly stable, and its abundance is transcriptionally controlled in the chorion laeve at term. Labor is not associated with changing PGHS-2 gene activity. Endogenous factors drive PGHS-2 gene transcription in the chorion, and the stable PGHS-2 mRNA accumulates in the tissue at term. This accumulation has little or no impact on the timing of labor.
- Subject
- chorion; PGHS-2 mRNA; term
- Identifier
- http://hdl.handle.net/1959.13/28895
- Identifier
- uon:2296
- Identifier
- ISSN:1071-5576
- Language
- eng
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