Localization of rRNA synthesis in Bacillus subtilis: characterization of loci involved in transcription focus formation

Title: Localization of rRNA synthesis in Bacillus subtilis: characterization of loci involved in transcription focus formation
Creator: Davies, Karen M.; Lewis, Peter J.
Relation: Journal of Bacteriology Vol. 185, Issue 7, p. 2346-2353
Publisher Link: http://dx.doi.org/10.1128/JB.185.7.2346-2353.2003
Publisher: American Society for Microbiology
Resource Type: journal article
Date: 2003
Description: In Bacillus subtilis, RNA polymerase becomes concentrated into regions of the nucleoid called transcription foci. With green fluorescent protein-tagged RNA polymerase, these structures are only observed at higher growth rates and have been shown to represent the sites of rRNA synthesis. There are 10 rRNA (rrn) operons distributed around nearly half of the chromosome. In this study we analyzed the rrn composition of transcription foci with fluorescently tagged loci and showed that they comprise the origin-proximal operon rrnO but not the more dispersed rrnE or rrnD. This suggests that transcription foci comprise only the seven origin-proximal operons rrnO, rrnA, rrnJ, rrnW, rrnI, rrnH, and rrnG. These results have important implications for our understanding of microbial chromosome structure.
Subject: Bacillus subtilis; RNA polymerase; transcription foci; microbial chromosome structure
Identifier: uon:1480
Identifier: http://hdl.handle.net/1959.13/27225
Identifier: ISSN:0021-9193
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