- Title
- Chironex fleckeri ( Box Jellyfish) venom proteins: Expansion of a Cnidarian toxin family that elicits variable cytolytic and cardiovascular effects
- Creator
- Brinkman, Diane L.; Konstantakopoulos, Nicki; McInerney, Bernie V.; Mulvenna, Jason; Seymour, Jamie E.; Isbister, Geoffrey K.; Hodgson, Wayne C.
- Relation
- NHMRC
- Relation
- Journal of Biological Chemistry Vol. 289, Issue 8, p. 4798-4812
- Publisher Link
- http://dx.doi.org/10.1074/jbc.M113.534149
- Publisher
- American Society for Biochemistry and Molecular Biology
- Resource Type
- journal article
- Date
- 2014
- Description
- The box jellyfish Chironex fleckeri produces extremely potent and rapid-acting venom that is harmful to humans and lethal to prey. Here, we describe the characterization of two C. fleckeri venom proteins, CfTX-A (∼40 kDa) and CfTX-B (∼42 kDa), which were isolated from C. fleckeri venom using size exclusion chromatography and cation exchange chromatography. Full-length cDNA sequences encoding CfTX-A and -B and a third putative toxin, CfTX-Bt, were subsequently retrieved from a C. fleckeri tentacle cDNA library. Bioinformatic analyses revealed that the new toxins belong to a small family of potent cnidarian pore-forming toxins that includes two other C. fleckeri toxins, CfTX-1 and CfTX-2. Phylogenetic inferences from amino acid sequences of the toxin family grouped CfTX-A, -B, and -Bt in a separate clade from CfTX-1 and -2, suggesting that the C. fleckeri toxins have diversified structurally and functionally during evolution. Comparative bioactivity assays revealed that CfTX-1/2 (25 μg kg−1) caused profound effects on the cardiovascular system of anesthetized rats, whereas CfTX-A/B elicited only minor effects at the same dose. Conversely, the hemolytic activity of CfTX-A/B (HU50 = 5 ng ml−1) was at least 30 times greater than that of CfTX-1/2. Structural homology between the cubozoan toxins and insecticidal three-domain Cry toxins (δ-endotoxins) suggests that the toxins have a similar pore-forming mechanism of action involving α-helices of the N-terminal domain, whereas structural diversification among toxin members may modulate target specificity. Expansion of the cnidarian toxin family therefore provides new insights into the evolutionary diversification of box jellyfish toxins from a structural and functional perspective.
- Subject
- bioinformatics; cardiovascular; endotoxin; protein purification; toxins; cubozoa; cytolytic; jellyfish toxin; nematocyst; venom
- Identifier
- http://hdl.handle.net/1959.13/1045897
- Identifier
- uon:14544
- Identifier
- ISSN:0021-9258
- Rights
- This research was originally published in Journal of Biological Chemistry. Brinkman, Diane L.; Konstantakopoulos, Nicki; McInerney, Bernie V.; Mulvenna, Jason; Seymour, Jamie E.; Isbister, Geoffrey K.; Hodgson, Wayne C. 'Chironex fleckeri ( Box Jellyfish) venom proteins: Expansion of a Cnidarian toxin family that elicits variable cytolytic and cardiovascular effects' Journal of Biological Chemistry Vol. 289, Issue 8, p. 4798-4812 © the American Society for Biochemistry and Molecular Biology
- Language
- eng
- Full Text
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