- Title
- Plasmacytoid dendritic cells and CD8 T cells from pregnant women show altered phenotype and function following H1N1/09 infection
- Creator
- Vanders, Rebecca L.; Gibson, Peter G.; Murphy, Vanessa E.; Wark, Peter A. B.
- Relation
- NHMRC.455592
- Relation
- Journal of Infectious Disease Vol. 208, Issue 7, p. 1062-1070
- Publisher Link
- http://dx.doi.org/10.1093/infdis/jit296
- Publisher
- Oxford University Press
- Resource Type
- journal article
- Date
- 2013
- Description
- Background: Pregnant women are a high-risk group during influenza pandemics. In this study we determined whether plasmacytoid dendritic cells (pDCs) and CD8 T cells from pregnant women display altered activity following in vitro infection with 2009 pandemic swine influenza (H1N1/09). Methods: Peripheral blood mononuclear cells (PBMCs) were isolated from pregnant (n = 26) and nonpregnant (n = 28) women. DC subtypes were enumerated from PBMCs. PBMCs were infected with H1N1/09 and CD86, human leukocyte antigen-DR (HLA-DR), and programmed death ligand 1/2 (PDL1/2) measured on pDCs. PD receptor 1 (PD1) was measured on CD8 T cells. Interferon-alpha (IFN-α), interleukin-2 (IL-2), tumor necrosis factor-alpha (TNFα), and IFN-gamma were measured from culture supernatant. Results: pDC (ie, CD303+/CD1c− PBMCs) percentages were lower in pregnant compared with nonpregnant women (P < .05). Following H1N1/09 infection, pDCs from pregnant women showed higher expression of CD86 (P < .01), HLA-DR (P < .001), and PDL1 (P < .001) compared with nonpregnant women. Expression of PD1 on CD8 T cells was higher during pregnancy (P < .05). Following H1N1/09 infection, PBMCs from pregnant women displayed reduced IFN-α (P < .01), IL-2 (P < .01), and IFN-γ (P < .01) compared with nonpregnant women. Blocking PDL1 during H1N1/09 infection increased these cytokines during pregnancy (P < .05). Conclusion: Altered maternal cellular antiviral activity is implicated in the increased morbidity during pregnancy following influenza pandemics.
- Subject
- CD8 T cell; H1N1/09; pDCs; pregnancy; PDL1; PDL2; CD86; HLA-DR; PD1; IFN
- Identifier
- http://hdl.handle.net/1959.13/1042919
- Identifier
- uon:14145
- Identifier
- ISSN:0022-1899
- Rights
- This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Journal of Infectious Disease following peer review. The definitive publisher-authenticated version is available online at: http://dx.doi.org/10.1093/infdis/jit296
- Language
- eng
- Full Text
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