- Title
- Modifier genes in Lynch syndrome: functional genomics and its consequence on disease expression
- Creator
- Reeves, Stuart
- Relation
- University of Newcastle Research Higher Degree Thesis
- Resource Type
- thesis
- Date
- 2012
- Description
- Research Doctorate - Doctor of Philosophy (PhD)
- Description
- Colorectal cancer (CRC) is globally a major cause of morbidity and mortality. Each year more than one million patients will be diagnosed with colorectal cancer, with about 15 - 20% of these patients having a family history or an inherited colorectal cancer syndrome. Somewhere between 1% and 7% (dependent on population under study) of these cases will have Lynch syndrome, which is the most common hereditary autosomal-dominant inherited cancer syndrome caused by germline mutations in deoxyribonucleic acid (DNA) mismatch repair genes. Patients diagnosed with Lynch syndrome who harbour a confirmed germline mutation in DNA mismatch repair (MMR) genes have an 80% lifetime risk of developing an epithelial malignancy. Each patient belongs to a family that requires special medical attention including genetic counselling, DNA testing for mismatch repair genes (most frequently hMLH1 or hMSH2) and screening for CRC. There is, however, considerable variation in the age of disease onset which is explained by a combination of genetic and environmental factors. The studies described in this thesis are aimed to better understand the genetic modifying effects on disease expression and how they relate to the likely age of colorectal cancer onset. Previous studies have identified a polymorphic CA repeat region in IGF-1 and two specific single nucleotide polymorphisms in MTHFR that were thought to alter the age of disease onset in individuals with Lynch syndrome. The effects of these polymorphisms were examined in larger multinational cohorts of patients and found to have significant effects on disease onset age. This is discussed in chapters 2, 3 and 4. Another similar study had identified a single nucleotide polymorphism in DNMT3B which was reported to have a significant effect in colorectal cancer expression in Lynch syndrome. The effect of this polymorphism was examined in a large multinational cohort of patients however, it was found to have no effect on the age of disease onset. Several candidate polymorphisms were also identified in the DNA repair genes BRCA2, hMSH3, Lig4, hOGG1, XRCC1, XRCC2 and XRCC3 but no significant associations were identified. The results from these studies are discussed in chapters 5 and 6. All data generated from these studies were extensively analysed by a combination of statistical tests that included Kaplan-Meier survival and Cox hazard regression analysis allowing data to be stratified by both single and multi variable factors. Allele frequencies were also tested for significant deviation from the Hardy-Weinberg equilibrium, while Pearson’s Chi-square test was utilised to evaluate differences in the allele frequencies between the multinational cohort groups and distribution of genotypes. The results described in this thesis contribute to a better understanding of disease expression in Lynch syndrome as it identifies genetic factors involved in the etiology of malignancy in this disease. The progress made in this area of medical research will aid in providing better predictive information of greater accuracy regarding the risks of colorectal cancer and enable the development of personalised cancer surveillance regimens.
- Subject
- Lynch syndrome; HNPCC; Colorectal cancer; genetics; thesis by publication
- Identifier
- http://hdl.handle.net/1959.13/929828
- Identifier
- uon:10691
- Rights
- Copyright 2012 Stuart Reeves
- Language
- eng
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| Thumbnail | File | Description | Size | Format | |||
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| View Details Download | ATTACHMENT01 | Abstract | 254 KB | Adobe Acrobat PDF | View Details Download | ||
| View Details Download | ATTACHMENT02 | Thesis | 8 MB | Adobe Acrobat PDF | View Details Download |