- Title
- Automatic cell segmentation in microscopic color images using ellipse fitting and watershed
- Creator
- Park, M.; Jin, J. S.; Peng, Y.; Summons, P.; Yu, D.; Cui, Y.; Luo, S.; Wang, F.; Santos, L.; Xu, M.
- Relation
- 2010 IEEE/ICME International Conference on Complex Medical Engineering (IEEE/ICME 2010). Proceedings of the IEEE/ICME International Conference on Complex Medical Engineering, CME2010 (Gold Coast, Qld 13-15 July, 2010) p. 69-74
- Publisher Link
- http://dx.doi.org/10.1109/ICCME.2010.5558869
- Publisher
- Institute of Electrical and Electronics Engineers (IEEE)
- Resource Type
- conference paper
- Date
- 2010
- Description
- This paper presents an efficient and innovative method for the automated counting of cells in a microscopic image. The performance of watershed-based algorithms for the segmentation of clustered cells has been well demonstrated. The strength of our algorithm lies in the fact that it incorporates knowledge of color in the image. Our method uses the watershed transform with iterative shape alignment and is shown to be more accurate in retaining cell shape. We report a sensitivity of 97% and specificity of 96% when all color bands are used. Our methods could be of value to computer-based systems designed to objectively interpret microscopic images, since they provide a means for accurate cell segmentation.
- Subject
- automatic cell segmentation; cell automated counting; cell shape; computer-based systems; watershed transform; watershed-based algorithms
- Identifier
- http://hdl.handle.net/1959.13/927220
- Identifier
- uon:10081
- Identifier
- ISBN:9781424468430
- Rights
- Copyright © 2010 IEEE. Reprinted from the Proceedings of the 2010 IEEE/ICME International Conference on Complex Medical Engineering. This material is posted here with permission of the IEEE. Such permission of the IEEE does not in any way imply IEEE endorsement of any of the University of Newcastle's products or services. Internal or personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution must be obtained from the IEEE by writing to pubs-permissions@ieee.org. By choosing to view this document, you agree to all provisions of the copyright laws protecting it.
- Language
- eng
- Full Text
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