https://nova.newcastle.edu.au/vital/access/manager/Index ${session.getAttribute("locale")} 5 https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:45260 Wed 26 Oct 2022 18:02:18 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:42962 Wed 22 Feb 2023 17:05:53 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:44188 Wed 19 Apr 2023 14:52:52 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:49349 Wed 17 Apr 2024 10:02:24 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:14485 Wed 11 Apr 2018 16:08:44 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:16821 Wed 11 Apr 2018 15:45:52 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:2527 Wed 11 Apr 2018 14:30:38 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:15133 Wed 11 Apr 2018 14:23:25 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:27712 Wed 11 Apr 2018 12:01:24 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:38419 Wed 08 Sep 2021 16:10:39 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:38138 Wed 04 Aug 2021 15:42:40 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:39108 Tue 10 May 2022 10:14:50 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:39106 Tue 10 May 2022 09:39:30 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:34948 Tue 03 Sep 2019 17:57:02 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:9651 Sat 24 Mar 2018 08:35:26 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:1294 Sat 24 Mar 2018 08:32:44 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:1032 Sat 24 Mar 2018 08:31:55 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:987 Sat 24 Mar 2018 08:29:49 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:1016 Sat 24 Mar 2018 08:29:47 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:2266 Sat 24 Mar 2018 08:26:55 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:17663 Sat 24 Mar 2018 07:57:48 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:6652 Sat 24 Mar 2018 07:46:20 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:38 Sat 24 Mar 2018 07:42:13 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:27976 50 = 6.7 nM) led to a significant reduction in dynamin phosphorylation (10.3% vs. 27.3%; P < 0.001), acrosomal exocytosis (9.7% vs. 25.7%; P < 0.01), and in vitro fertilization (53% vs. 100%; P < 0.01). GSK3 was shown to be present in developing germ cells where it colocalized with dynamin in the peri-acrosomal domain. However, additional GSK3 was acquired by maturing mouse spermatozoa within the male reproductive tract, via a novel mechanism involving direct interaction of sperm heads with extracellular structures known as epididymal dense bodies. These data reveal a novel mode for the cellular acquisition of a protein kinase and identify a key role for GSK3 in the regulation of sperm maturation and acrosomal exocytosis.—Reid, A. T., Anderson, A. L., Roman, S. D., McLaughlin, E. A., McCluskey, A., Robinson, P. J., Aitken, R. J., Nixon, B. Glycogen synthase kinase 3 regulates acrosomal exocytosis in mouse spermatozoa via dynamin phosphorylation.]]> Sat 24 Mar 2018 07:38:43 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:27718 via the enzyme, cytochrome P450, family 2, subfamily E, polypeptide 1 (CYP2E1). Acrylamide was administered to male CD1 mice for three or sixmonths at a dose of 0.18 mg/kg bodyweight/day. DNA damage was detected in germ cells and maturespermatozoa of exposed mice without compromising their overall fertility. The use of resveratrol, anantioxidant and known CYP2E1 inhibitor, was found to ameliorate the DNA damage in both germ cells andspermatozoa. However, extended resveratrol treatment (six months, 10.0 mg/kg bw/week) resulted inpremature activation of these cells. Thus the DNA damage found in spermatozoa after chronic acrylamideadministration can be alleviated but an alternative CYP2E1 inhibitor may be required.]]> Sat 24 Mar 2018 07:24:39 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:27719 Sat 24 Mar 2018 07:24:39 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:26537 95% of sperm. Anapparent molecular rearrangement in the complex occurs, bringing PRKACA and TCP11 into proximity. Taken together, these results suggest a role for the novel complex of SPIF, PRKACA, and TCP11 during spermcapacitation, fertilization, and embryogenesis.]]> Sat 24 Mar 2018 07:23:27 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:22263 in vitro induction of acrosomal exocytosis by progesterone, but not by the calcium ionophore A23187, and elicited a concomitant reduction of in vitro fertilization. In vivo treatment with these inhibitors also resulted in spermatozoa displaying reduced acrosome reaction potential. Dynamin 1 and 2 phosphorylation increased on progesterone treatment, and this was also selectively blocked by dynasore. On the basis of our collective data, we propose that dynamin could regulate specific membrane fusion events necessary for acrosomal exocytosis in mouse spermatozoa.]]> Sat 24 Mar 2018 07:17:38 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:25108 Sat 24 Mar 2018 07:17:15 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:25107 Sat 24 Mar 2018 07:17:06 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:46833 Mon 29 Jan 2024 18:41:10 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:54975 Mon 25 Mar 2024 15:21:41 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:34117 Mon 11 Feb 2019 16:05:21 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:51402 Mon 04 Sep 2023 14:52:16 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:40281 Fri 29 Jul 2022 13:23:12 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:45427 Fri 28 Oct 2022 11:48:22 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:49362 Fri 12 May 2023 12:35:13 AEST ]]>