https://nova.newcastle.edu.au/vital/access/manager/Index ${session.getAttribute("locale")} 5 https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:49984 Wed 28 Feb 2024 15:55:05 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:50571 Wed 28 Feb 2024 15:53:55 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:51503 Wed 28 Feb 2024 14:50:10 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:39903 Wed 22 Mar 2023 10:31:41 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:43949 Wed 05 Oct 2022 12:58:31 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:34255 Ex situ analyses of human myometrial tissue has been used to investigate the regulation of uterine quiescence and transition to a contractile phenotype. Following concerns about the validity of cultured primary cells, we examined whether myometrial tissue undergoes culture-induced changes ex situ that may affect the validity of in vitro models. Objectives: To determine whether human myometrial tissue undergoes culture-induced changes ex situ in Estrogen receptor 1 (ESR1), Prostaglandin-endoperoxide synthase 2 (PTGS2) and Oxytocin receptor (OXTR) expression. Additionally, to determine whether culture conditions approaching the in vivo environment influence the expression of these key genes. Methods: Term non-laboring human myometrial tissues were cultured in the presence of specific treatments, including; serum supplementation, progesterone and estrogen, cAMP, PMA, stretch or NF-κB inhibitors. ESR1, PTGS2 and OXTR mRNA abundance after 48 h culture was determined using quantitative RT-PCR. Results: Myometrial tissue in culture exhibited culture-induced up-regulation of ESR1 and PTGS2 and down-regulation of OXTR mRNA expression. Progesterone prevented culture-induced increase in ESR1 expression. Estrogen further up-regulated PTGS2 expression. Stretch had no direct effect, but blocked the effects of progesterone and estrogen on ESR1 and PTGS2 expression. cAMP had no effect whereas PMA further up-regulated PTGS2 expression and prevented decline of OXTR expression. Conclusion: Human myometrial tissue in culture undergoes culture-induced gene expression changes consistent with transition toward a laboring phenotype. Changes in ESR1, PTGS2 and OXTR expression could not be controlled simultaneously. Until optimal culture conditions are determined, results of in vitro experiments with myometrial tissues should be interpreted with caution.]]> Wed 04 Sep 2019 10:05:05 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:31201 Wed 02 Mar 2022 14:27:45 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:41102 Tue 26 Jul 2022 14:20:11 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:33374 Tue 23 Oct 2018 13:32:38 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:50315 Tue 18 Jul 2023 12:37:57 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:19823 Tue 09 Jun 2020 09:48:40 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:45207 Rhodiola wallichiana var. cholaensis (RWC) has the potential to alleviate asthmatic inflammation according to recent studies, but its pharmacological mechanisms remain unclarified. In our study, murine asthmatic phenotypes were established and treated with RWC and/or dexamethasone (DEX). Combined treatment with RWC and DEX could improve spirometry and airway hyperresponsiveness (AHR) in asthmatic phenotypes, alleviate steroid resistance in NEA, and reduce the inflammatory infiltration of the both phenotypes. The combined treatment increased Th1, regulated the imbalance of Th2/Th1, and decreased the related cytokines in EA. As for NEA, the combined treatment reduced Th17 and promoted the accumulation of regulatory T cells (Tregs) in lung. A microbiome study based on 16S rDNA sequencing technique revealed the significantly changed structure of the lower airway microbiota after combined treatment in NEA, with 4 distinct genera and 2 species identified. OPLS-DA models of metabolomics analysis based on UPLC-Q/TOF-MS technique identified 34 differentiated metabolites and 8 perturbed metabolic pathways. A joint multiomics study predicted that the colonized microbiota in airways might be associated with susceptibility of asthma and steroid resistance, which involved systematic and pulmonary metabolic perturbation. In summary, the pharmacological network of RWC included the complicated interaction mechanisms of immune regulation, microbiota change, and metabolic perturbation.]]> Thu 27 Oct 2022 15:07:49 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:375 Thu 25 Jul 2013 09:10:05 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:154 g and Nt540c>t, which have been detected in other populations. To establish if they are associated with an increased malignant melanoma (MM) risk we did an association study based on genotyping 471 patients with MM and 1,2 10 random control subjects from the same Polish population. We found a significantly increased frequency of the A148T variant among patients with MM (7.0%) in comparison with the general population (2.9%). The incidence of the A148T variant remained greater in both unselected and familial melanoma subgroups. A statistically significant positive association was seen for unselected MM (odds ratio, 2.529; P = 0.0003), especially in patients diagnosed under 50 years of age (odds ratio, 3.4; P = 0.0002). The A148T carrier population (heterozygous G/A alleles) was more likely to have a relative with malignancy compared with the noncarrier population (57% versus 36%, respectively; P = 0.03). Further examination of the CDKN2A promoter sequence done in 20 melanoma patients with the A148T change (heterozygous G/A alleles) and 20 patients with MM without this alteration identified it was in linkage disequilibrium with a polymorphism in the promoter region at position P-493. We found no statistically significant overrepresentation of the Nt500c>g and the Nt540c>t polymorphisms in the Polish melanoma population. In conclusion, the A148T variant of the CDKN2A gene seems to be associated with an increased risk of development of MM. Additional studies are required to confirm whether this particular change is associated with increased risk of other nonmelanoma malignancies.]]> Thu 25 Jul 2013 09:09:40 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:24852 Thu 17 Feb 2022 09:28:28 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:7490 Sat 24 Mar 2018 08:37:45 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:8198 Sat 24 Mar 2018 08:36:19 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:13321 Sat 24 Mar 2018 08:16:41 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:17886 Sat 24 Mar 2018 07:56:18 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:20329 Sat 24 Mar 2018 07:55:11 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:5556 Sat 24 Mar 2018 07:49:12 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:5093 Sat 24 Mar 2018 07:48:50 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:5230 Sat 24 Mar 2018 07:44:18 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:4993 A/3111-1G>A. Two cases had no gastrointestinal symptoms or signs of PA. PA is an inconstant feature of the subtype of epidermolysis bullosa known as JEB-PA. It is most likely that multiple factors influence the development of PA and its presence is not predictive of a poor outcome. It is possible that institutions that do not routinely screen immunofluorescence mapping for integrin α6β4 staining in the absence of PA are missing this form of epidermolysis bullosa.]]> Sat 24 Mar 2018 07:44:15 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:29214 Sat 24 Mar 2018 07:36:47 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:38984 9/L and FENO ≤29 ppb. Exacerbations requiring medical intervention were recorded. Results: Among 220 non-smokers (n = 109 control, n = 111 FENO), 1006 treatment decisions were made, with significant group differences after the first and second algorithm applications. 53% of women had NEA. Treatment was better targeted to phenotype in the FENO group: ICS use increased in eosinophilic asthma (EA, 48–86%), while ICS/LABA increased in NEA (11–30%). Fewer women in the FENO group had exacerbations during pregnancy in NEA only (18.9% FENO vs 44% control, P = 0.006). Conclusion: The FENO algorithm was more effective in treating NEA, resulting in reduced exacerbations, compared to a symptom control algorithm. This was not the result of ICS overtreatment, since the benefits occurred at a lower median daily ICS dose. Two applications of the FENO-guided algorithm, one month apart, were sufficient to achieve beneficial effects in terms of asthma exacerbations, among pregnant women with asthma.]]> Mon 29 Jan 2024 17:52:03 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:47306 Fri 13 Jan 2023 10:52:32 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:26225 Fri 03 Dec 2021 10:33:00 AEDT ]]>