https://nova.newcastle.edu.au/vital/access/manager/Index ${session.getAttribute("locale")} 5 https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:51294 Wed 30 Aug 2023 13:37:21 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:51223 Wed 28 Feb 2024 15:37:01 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:39575 Wed 27 Jul 2022 14:43:55 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:39552 Wed 27 Jul 2022 14:30:46 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:40464 Wed 27 Jul 2022 13:10:26 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:45105 Wed 26 Oct 2022 13:21:01 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:55187 Wed 24 Apr 2024 10:02:08 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:42046 Wed 17 Aug 2022 13:44:19 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:13047 Wed 11 Apr 2018 12:21:31 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:12048 Wed 11 Apr 2018 11:01:28 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:19565 Wed 11 Apr 2018 10:55:18 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:46708 Wed 07 Feb 2024 18:00:11 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:43966 Wed 05 Oct 2022 14:15:21 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:52141 Wed 04 Oct 2023 09:53:05 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:54436 Tue 27 Feb 2024 13:49:20 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:13860 Tue 24 Aug 2021 14:00:15 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:53960 Tue 23 Jan 2024 12:38:46 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:55172 Tue 23 Apr 2024 09:38:58 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:52539 Tue 17 Oct 2023 10:12:32 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:41993 6 s (sensitivity, 88%; specificity, 92%). The computed tomographic perfusion collateral index, defined by the ratio of delay time >6 s/delay time >2 s volume, showed a significant correlation with dynamic computed tomographic angiography collateral scores (correlation coefficient, 0.62; P<0.001), with an optimal cut point of 31.8% in predicting good collateral status (sensitivity of 83% and specificity of 86%). When predicting good clinical outcome, the delay time collateral index showed a similar predictive power to dynamic computed tomographic angiography collaterals (area under the curve, 0.78 [0.67–0.83] and 0.77 [0.69–0.84], respectively; P<0.001). Conclusions—Computed tomographic perfusion can accurately quantify collateral flow after acute ischemic stroke.]]> Tue 16 Aug 2022 16:37:23 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:45941 Tue 08 Nov 2022 10:07:09 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:40146 Tue 05 Jul 2022 15:49:42 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:41392 Tue 02 Aug 2022 17:47:29 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:41383 Tue 02 Aug 2022 15:48:38 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:50654 Tue 01 Aug 2023 12:10:37 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:39913 Ascomycete sp. F53 (F53), a fungal endophyte of the traditional Chinese medicinal plant Taxus yunnanensis (Chinese yew), revealed 35 putative specialized metabolite biosynthesis gene clusters, one of which encodes a rarely seen tandem polyketide synthase pathway with close homology to azaphilone biosynthesis pathways. A novel compound, lijiquinone 1, was subsequently isolated from F53 and structurally and functionally characterized. The m/z 385 [M + H⁺]⁺ compound, comprised of a cyclohexenone side group attached to a core bicyclic ring, displayed cytotoxicity against human myeloma cells (IC₅₀ = 129 μM), as well as antifungal activity against Candida albicans (IC₅₀ = 79 μM) and Cryptococcus albidus (IC₅₀ = 141 μM). Our results suggest that enzymes encoded on the lij gene cluster are responsible for the synthesis of 1 and that the medicinal properties of T. yunnanensis could be partially mediated by this novel azaphilone. This study highlights the utility of combining traditional knowledge with contemporary genomic approaches for the discovery of new bioactive compounds.]]> Thu 30 Jun 2022 09:56:32 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:55006 Thu 28 Mar 2024 13:52:11 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:53994 Thu 25 Jan 2024 13:10:42 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:51168 Thu 24 Aug 2023 14:09:35 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:40967 Thu 21 Jul 2022 08:38:13 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:35902 Thu 20 Feb 2020 11:02:51 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:50862 Thu 17 Aug 2023 11:36:01 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:43283 Thu 15 Sep 2022 12:30:39 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:40073 Thu 14 Jul 2022 13:32:13 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:53642 Thu 14 Dec 2023 09:39:51 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:37449 Thu 14 Apr 2022 11:00:25 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:49209 Sun 07 May 2023 09:29:54 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:50801 Sun 06 Aug 2023 09:12:28 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:41252 Sat 30 Jul 2022 12:54:21 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:14873 Sat 24 Mar 2018 08:22:23 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:18462 3% and > 61% respectively. Peripheral blood monocytes were isolated (n = 61) and sputum macrophages were isolated from a subgroup of patients with asthma (n = 14), using immunomagnetic cell separation. RNA and nuclear proteins were extracted and quantified. Enzyme activity was assessed using fluorescent assays and gene expression of EP300, KAT2B, CREBBP, and HDACs 1, 2 and 3 were measured by qPCR. Results: There was a significant inverse association between blood monocyte HAT and HDAC activity (r = −0.58, P < 0.001). NA was associated with increased blood monocyte HAT enzyme activity (P = 0.02), decreased HDAC activity (P = 0.03), and increased HAT: HDAC ratio (P < 0.01) compared with eosinophilic asthma. There were no differences in gene expression of EP300, KAT2B, CREBBP, or HDACs 1, 2 and 3 in blood monocytes from subjects with asthma or inflammatory phenotypes of asthma. There was no effect of inhaled corticosteroid use, poor asthma control, or asthma severity on HAT/HDAC activities. Sputum macrophages had increased expression of KAT2B in eosinophilic compared with paucigranulocytic asthma. Conclusions and Clinical Relevance: Neutrophilic airway inflammation is associated with increased HAT and reduced HDAC activity in blood monocytes, demonstrating further systemic manifestations relating to the altered inflammatory gene transcription profile of neutrophilic asthma.]]> Sat 24 Mar 2018 07:59:46 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:21808 Sat 24 Mar 2018 07:59:22 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:21650 Cryptosporidium is an enteric parasite of public health significance that causes diarrhoeal illness through faecal oral contamination and via water. Zoonotic transmission is difficult to determine as most species of Cryptosporidium are morphologically identical and can only be differentiated by molecular means. Transmission dynamics of Cryptosporidium in rural populations were investigated through the collection of 196 faecal samples from diarrheic (scouring) calves on 20 farms and 63 faecal samples from humans on 14 of these farms. The overall prevalence of Cryptosporidium in cattle and humans by PCR and sequence analysis of the 18S rRNA was 73.5% (144/196) and 23.8% (15/63), respectively. Three species were identified in cattle; Cryptosporidium parvum, Cryptosporidium bovis and Cryptosporidium ryanae, and from humans, C. parvum and C. bovis. This is only the second report of C. bovis in humans. Subtype analysis at the gp60 locus identified C. parvum subtype IIaA18G3R1 as the most common subtype in calves. Of the seven human C. parvum isolates successfully subtyped, five were IIaA18G3R1, one was IIdA18G2 and one isolate had a mix of IIaA18G3R1 and IIdA19G2. These findings suggest that zoonotic transmission may have occurred but more studies involving extensive sampling of both calves and farm workers are needed for a better understanding of the sources of Cryptosporidium infections in humans from rural areas of Australia.]]> Sat 24 Mar 2018 07:52:23 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:6854 Sat 24 Mar 2018 07:47:40 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:196 Sat 24 Mar 2018 07:42:54 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:35421 Mon 29 Jul 2019 16:00:33 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:18939 Mon 27 Jul 2015 11:51:12 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:18921 Mon 27 Jul 2015 09:03:12 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:47552 Mon 23 Jan 2023 12:29:50 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:51101 Mon 21 Aug 2023 14:46:33 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:39532 Mon 08 Aug 2022 11:20:12 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:4348 Mon 08 Apr 2019 09:49:35 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:51402 Mon 04 Sep 2023 14:52:16 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:45409 Fri 28 Oct 2022 14:36:32 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:33965 Fri 25 Jan 2019 14:42:22 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:51909 Fri 22 Sep 2023 10:25:28 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:35366 Fri 19 Jul 2019 09:29:25 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:53194 Fri 17 Nov 2023 11:51:52 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:51712 Fri 15 Sep 2023 14:23:03 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:51707 Fri 15 Sep 2023 14:09:33 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:39992 p < 0.001). Many survivors and parents have unmet needs for genetics-related services and information. Greater access to services and information might allow survivors at high risk for late effects to detect and prevent side effects early and improve medical outcomes. Addressing families’ needs and preferences during survivorship may increase satisfaction with survivorship care.]]> Fri 15 Jul 2022 10:13:46 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:49414 Fri 12 May 2023 15:02:09 AEST ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:46098 Fri 11 Nov 2022 11:26:33 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:46089 Fri 11 Nov 2022 10:47:20 AEDT ]]> https://nova.newcastle.edu.au/vital/access/manager/Repository/uon:49202 Fri 05 May 2023 15:58:01 AEST ]]>