http://novaprd-lb.newcastle.edu.au/vital/access/manager/Index ${session.getAttribute("locale")} 5 Fluoxetine prevents development of an early stress-related molecular signature in the rat infralimbic medial prefrontal cortex. Implications for depression? http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15159 Wed 17 Sep 2014 16:25:19 EST ]]> A cross-sectional study assessing the self-reported weight loss strategies used by adult Australian general practice patients http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15158 Wed 17 Sep 2014 16:18:21 EST ]]> Study protocol: the registrar clinical encounters in training (ReCEnT) study http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15157 Wed 17 Sep 2014 16:04:20 EST ]]> Wavelet signatures of climate and flowering: identification of species groupings http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15156 Wed 17 Sep 2014 15:50:19 EST ]]> Efficacy of manual therapy treatments for people with cervicogenic dizziness and pain: protocol of a randomised controlled trial http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15155 Wed 17 Sep 2014 15:43:20 EST ]]> Efficacy of acupuncture for chronic knee pain: protocol for a randomised controlled trial using a Zelen design http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15154 Wed 17 Sep 2014 15:29:20 EST ]]> Numerical modelling of ground temperature evolution and soil subsidence as a result of underground coal fire http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15152 Wed 17 Sep 2014 15:08:19 EST ]]> Wearing face masks in public during the influenza season may reflect other positive hygiene practices in Japan http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15153 Wed 17 Sep 2014 15:08:19 EST ]]> Comparative in-vivo toxicity of venoms from South Asian hump-nosed pit vipers (Viperidae: Crotalinae: Hypnale) http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15146 Wed 17 Sep 2014 13:34:24 EST ]]> In situ rockfall testing in New South Wales, Australia http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:13856 n and kt) and the equivalent rolling coefficient (μ), which are site-specific. Several series of rockfall tests have been conducted in three different geological environments in New South Wales. The results of the tests show a large variability of the motion parameters, which is due to the natural variability of the blocks and to the randomness of the impact positions. Also, values of kn consistently and systematically higher than the benchmark values from the literature have been inferred. Despite there being no clear correlation between the restitution coefficients and the rotational energy, rotational phenomena are believed to be at the origin of such results.]]> Wed 17 Sep 2014 13:33:06 EST ]]> Pertussis vaccination in child care workers: room for improvement in coverage, policy and practice http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15151 Wed 17 Sep 2014 13:23:20 EST ]]> Wavelet signatures and diagnostics for the assessment of ICU agitation-sedation protocols http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15150 Wed 17 Sep 2014 13:09:19 EST ]]> Socioeconomic inequality in domains of health: results from the World Health Surveys http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15149 Wed 17 Sep 2014 13:09:19 EST ]]> An olfactory 'stress test' may detect preclinical Alzheimer's disease http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15148 Wed 17 Sep 2014 12:48:19 EST ]]> Ser/thr phosphatases: the new frontier for myeloid leukemia therapy? http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15147 Wed 17 Sep 2014 12:41:19 EST ]]> A phase II clinical trial of a dental health education program delivered by aboriginal health workers to prevent early childhood caries http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15145 Wed 17 Sep 2014 12:13:20 EST ]]> An investigation of factors associated with the health and well-being of HIV-infected or HIV-affected older people in rural South Africa http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15144 Wed 17 Sep 2014 12:06:20 EST ]]> Embedding chiropractic in indigenous health care organisations: applying the normalisation process model http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15143 Wed 17 Sep 2014 11:52:19 EST ]]> Still waiting for Nike to do it : Nike's labor practices in the three years since CEO Phil Knight's speech to the National Press Club http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15142 Wed 17 Sep 2014 11:28:48 EST ]]> Still life moments http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:6341 Wed 17 Sep 2014 11:10:17 EST ]]> Loss of secreted frizzled-related protein 4 correlates with an aggressive phenotype and predicts poor outcome in ovarian cancer patients http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15140 Wed 17 Sep 2014 08:50:19 EST ]]> RAB-Like 2 has an essential role in male fertility, sperm intra-flagellar transport, and tail assembly http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15139 Tue 16 Sep 2014 16:09:19 EST ]]> GPU-FS-kNN: a software tool for fast and scalable kNN computation using GPUs http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15138 Tue 16 Sep 2014 13:00:19 EST ]]> Unveiling clusters of RNA transcript pairs associated with markers of Alzheimer's disease progression http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15137 Tue 16 Sep 2014 12:32:20 EST ]]> Injectable and oral contraceptive use and cancers of the breast, cervix, ovary, and endometrium in black South African women: case-control study http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15136 Tue 16 Sep 2014 11:50:20 EST ]]> Polymyxin B inadequately quenches the effects of contaminating lipopolysaccharide on murine dendritic cells http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15135 Tue 16 Sep 2014 11:29:19 EST ]]> Social determinants of self-reported health in women and men: understanding the role of gender in population health http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15134 Tue 16 Sep 2014 11:08:20 EST ]]> A unique combination of male germ cell mirnas coordinates gonocyte differentiation http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15133 Tue 16 Sep 2014 10:33:20 EST ]]> Critical role of constitutive type I interferon response in bronchial epithelial cell to influenza infection http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15132 Tue 16 Sep 2014 10:12:19 EST ]]> Effect of low temperature on growth and ultra-structure of staphylococcus spp http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15131 Tue 16 Sep 2014 09:44:20 EST ]]> Effect of maternal probiotic intervention on HPA Axis, immunity and gut microbiota in a rat model of irritable bowel syndrome http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15130 Tue 16 Sep 2014 09:23:19 EST ]]> Who uses glucosamine and why? A study of 266,848 Australians aged 45 years and older http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15129 Tue 16 Sep 2014 08:55:20 EST ]]> Ionic liquids as porogens in the synthesis of molecularly imprinted polymers http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15165 Thu 18 Sep 2014 16:06:19 EST ]]> Large scale tests on the expansiveness of resin-injected clay http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15163 Thu 18 Sep 2014 14:42:19 EST ]]> Design parameters for rockfall protection barriers in the rocks of the Narrabeen Group http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15162 Thu 18 Sep 2014 12:57:20 EST ]]> Genome-wide association and functional follow-up reveals new loci for kidney function http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15141 Thu 18 Sep 2014 12:29:17 EST ]]> Perforation of rockfall protection barriers by normal block impact http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15161 Thu 18 Sep 2014 11:54:18 EST ]]> Monitoring of an encapsulated embankment for the validation of a dimensionless model for soil swelling http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:14086 Thu 18 Sep 2014 11:19:50 EST ]]> Management of reactive and carbonaceous materials in the earthworks design for the Hunter Expressway, Minmi to Buchanan section http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:14087 Thu 18 Sep 2014 11:17:53 EST ]]> Numerical modeling of a rock fall mesh perforation upon impact http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:13204 Thu 18 Sep 2014 11:12:19 EST ]]> Use of hand-spray plaster as a coating for soil bulk volume measurement http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15160 Thu 18 Sep 2014 10:58:17 EST ]]> An indirect approach for correlation of permeability and diffusion coefficients http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:7799 Thu 18 Sep 2014 10:37:35 EST ]]> Laboratory investigation on high values of restitution coefficients http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:13855 n are below unity. Values greater than one are quite rare, seen as unusual and barely explained. Previous experimental research conducted in Australia has shown consistent and systematic values of the normal restitution coefficient greater than one. This was tentatively explained by a combination of parameters such as low impacting angle, rotational energy and block angularity. The study presented in this paper aims at (1) identifying the critical parameters conducting to high kn values and (2) at explaining the associated motion mechanisms. The objective was reached with values of kn up to almost 2. In addition, the study has confirmed the significance of low impacting angle, rotational energy and block shape in this context.]]> Thu 18 Sep 2014 09:50:41 EST ]]> Prediction of the bullet effect for rockfall barriers: a scaling approach http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:13857 Thu 18 Sep 2014 09:48:03 EST ]]> New insight into cavitation mechanisms in high-capacity tensiometers based on high-speed photography http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:13854 Thu 18 Sep 2014 09:40:06 EST ]]> Perforation of flexible rockfall barriers by normal block impact http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:13853 Thu 18 Sep 2014 09:25:23 EST ]]> Installation, calibration, and application of dielectric sensors in laboratory studies of compacted unsaturated granular materials http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:13852 3/m3. To demonstrate the potential of dielectric sensors for use in the study of compacted granular materials, two laboratory experiments are described: one monitoring changes in moisture content with time and the other changes in electrical conductivity. General recommendations for use of dielectric sensors with granular materials are also presented.]]> Thu 18 Sep 2014 08:51:58 EST ]]> The nature of things: an interdisciplanary investigation into the experiences and impacts of drought for three generations of Australian women http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15180 Mon 22 Sep 2014 16:14:17 EST ]]> From Southern Africa to the Great Lakes Region: challenges to Tanzanian foreign policy and conflict resolution in Sub-Saharan Africa http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15179 Mon 22 Sep 2014 16:00:21 EST ]]> The contribution of genetic susceptibility to breast cancer http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15177 Mon 22 Sep 2014 15:53:20 EST ]]> Analysis of post-translational modifications of Fat1 cadherin http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15178 Mon 22 Sep 2014 15:53:20 EST ]]> A cluster randomised trial of a school-based resilience intervention to decrease tobacco, alcohol and illicit drug use in secondary school students: study protocol http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15190 Mon 22 Sep 2014 15:32:19 EST ]]> Towards a dimensionless description of soil swelling behaviour http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15189 w, was specifically derived and validated using datasets from the literature. These data were obtained from monotonic one-dimensional swelling tests until full saturation. The results suggest that dimensional analysis and the resulting dimensionless model can be used to predict soil swelling with relatively good accuracy.]]> Mon 22 Sep 2014 14:57:19 EST ]]> Can exercise ameliorate treatment toxicity during the initial phase of testosterone deprivation in prostate cancer patients? Is this more effective than delayed rehabilitation? http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15188 Mon 22 Sep 2014 14:50:19 EST ]]> Ageing in general practice (AGP) trial: a cluster randomised trial to examine the effectiveness of peer education on GP diagnostic assessment and management of dementia http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15187 Mon 22 Sep 2014 14:29:19 EST ]]> Impact of behavioural risk factors on death within 10 years for women and men in their 70s: absolute risk charts http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15184 Mon 22 Sep 2014 14:22:17 EST ]]> A fractal basis for soil-water characteristics curves with hydraulic hysteresis http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15186 Mon 22 Sep 2014 14:05:00 EST ]]> An experimental study of salt movement and accumulation within unsaturated granular road pavements http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15164 Mon 22 Sep 2014 13:12:19 EST ]]> The quality of the evidence base for clinical pathway effectiveness: room for improvement in the design of evaluation trials http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15185 Mon 22 Sep 2014 13:05:19 EST ]]> Martial and domitians moral reforms http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15169 Mon 22 Sep 2014 12:58:18 EST ]]> Effect of naturally occurring salts on tensile and shear strength of sealed granular road pavements http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15168 Mon 22 Sep 2014 12:58:17 EST ]]> Retention of allied health professionals in rural New South Wales: a thematic analysis of focus group discussions http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15183 Mon 22 Sep 2014 12:30:19 EST ]]> Poor food and nutrient intake among Indigenous and non-Indigenous rural Australian children http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15182 Mon 22 Sep 2014 12:09:19 EST ]]> Changes in the bird population of Bolwarra, 1994-2011 http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15181 Mon 22 Sep 2014 12:02:21 EST ]]> Growing old before growing rich: inequality in health service utilization among the mid-aged and elderly in Gansu and Zhejiang Provinces, China http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15176 Mon 22 Sep 2014 11:27:19 EST ]]> Breakingtheice: a protocol for a randomised controlled trial of an internet-based intervention addressing amphetamine-type stimulant use http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15175 Mon 22 Sep 2014 10:10:19 EST ]]> Can personal qualities of medical students predict in-course examination success and professional behaviour? An exploratory prospective cohort study http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15174 Mon 22 Sep 2014 09:56:19 EST ]]> A cluster-randomized controlled trial of strategies to increase adolescents' physical activity and motivation during physical education lessons: the Motivating Active Learning in Physical Education (MALP) trial http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15173 Mon 22 Sep 2014 09:35:20 EST ]]> A parallel genome-wide mRNA and microRNA profiling of the frontal cortex of HIV patients with and without HIV-associated dementia shows the role of axon guidance and downstream pathways in HIV-mediated neurodegeneration http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15172 Mon 22 Sep 2014 09:14:19 EST ]]> Parent attitudes, family dynamics and adolescent drinking: qualitative study of the Australian parenting guidelines for adolescent alcohol use http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15171 Mon 22 Sep 2014 09:00:19 EST ]]> A qualitative study into the use of formal services for dementia by carers from culturally and linguistically diverse (CALD) communities http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15170 Mon 22 Sep 2014 08:39:19 EST ]]> Systemic inflammation in obstructive airway disease http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15127 Mon 15 Sep 2014 17:03:21 EST ]]> Low pathogenic human enteroviruses as novel anti-cancer agents against malignant glioma http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15125 Mon 15 Sep 2014 17:03:17 EST ]]> Tyrosine hydroxylase phosphorylation in catecholaminergic brain regions: a marker of activation following acute hypotension and glucoprivation http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15128 Mon 15 Sep 2014 16:07:20 EST ]]> In pursuit of motion: developing a methodological framework for illustrating moments of movement in birds and fish http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15124 Mon 15 Sep 2014 15:46:21 EST ]]> Structural barriers to timely initiation of antiretroviral treatment in Vietnam: findings from six outpatient clinics http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15126 Mon 15 Sep 2014 15:25:19 EST ]]> The role of microRNAs in allergic airways disease and T cell biology http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15123 Mon 15 Sep 2014 15:11:19 EST ]]> The molecular chaperone HSPA2 plays a key role in regulating the expression of sperm surface receptors that mediate sperm-egg recognition http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15122 Mon 15 Sep 2014 15:11:19 EST ]]> Stromal liver kinase B1 STK11 signaling loss induces oviductal adenomas and endometrial cancer by activating mammalian target of Rapamycin Complex 1 http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15121 Mon 15 Sep 2014 14:50:19 EST ]]> Multivariate protein signatures of pre-clinical Alzheimer's disease in the Alzheimer's disease meuroimaging initiative (ADNI) plasma proteome dataset http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15120 Mon 15 Sep 2014 12:58:19 EST ]]> Stress-induced susceptibility to sudden cardiac death in mice with altered serotonin homeostasis http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15119 Mon 15 Sep 2014 12:37:19 EST ]]> Transcriptome sequencing revealed significant alteration of cortical promoter usage and splicing in schizophrenia http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15118 Mon 15 Sep 2014 12:16:21 EST ]]> Religious experience and women leadership in Nigerian Islam: a case study of Alhaja Sheidat Mujidat Adeoye http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15117 Mon 15 Sep 2014 12:16:19 EST ]]> Editorial http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15116 Mon 15 Sep 2014 12:09:25 EST ]]> Safety culture within the Australian coal mining industry: identifying the critical factors and measuring the construct http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:14914 Mon 15 Sep 2014 11:34:21 EST ]]> Book review: Reinventing the Male Homosexual: the Rhetoric and Power of the Gay Gene http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15112 Mon 15 Sep 2014 11:34:20 EST ]]> Book review: Girls Feminine Adolescence in Popular Culture and Cultural Theory http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15113 Mon 15 Sep 2014 11:34:19 EST ]]> Book review: Manning the Next Millennium Studies in Masculinities http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15114 Mon 15 Sep 2014 11:34:18 EST ]]> Book review: Women in African Colonial Histories http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15111 Mon 15 Sep 2014 11:34:18 EST ]]> Sodium chloride inhibits the growth and infective capacity of the amphibian chytrid fungus and increases host survival rates http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15115 Mon 15 Sep 2014 11:20:20 EST ]]> MicroRNA-16 is down-regulated in mutated FLT3 expressing murine myeloid FDC-P1 cells and interacts with Pim-1 http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15110 Mon 15 Sep 2014 10:52:23 EST ]]> Book review: Chinese Femininities Chinese Masculinities: A Reader http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15109 Mon 15 Sep 2014 10:52:20 EST ]]> Book review: Images of the 'Modern Woman' in Asia: Global Media, Local Meanings http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15107 Mon 15 Sep 2014 10:45:26 EST ]]> Book review: May Her Likes be Multiplied: Biography and Gender Politics in Egypt http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15108 Mon 15 Sep 2014 10:45:19 EST ]]> Drosophila Rbp6 is an orthologue of vertebrate Msi-1 and Msi-2, but does not function redundantly with dMsi to regulate germline stem cell behaviour http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15105 Mon 15 Sep 2014 10:38:24 EST ]]> Book review: Gender Trouble Down Under: Australian Masculinities http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15106 Mon 15 Sep 2014 10:38:21 EST ]]> Book review: Liberty for Women, Freedom and Feminism in the Twenty-First Century http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15104 Mon 15 Sep 2014 10:31:26 EST ]]> Book review: From Camp to Queer: re-making the Australian Homosexual http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15103 Mon 15 Sep 2014 10:24:19 EST ]]> Evidence for upregulation of Bim and the splicing factor SRp55 in melanoma cells from patients treated with selective BRAF inhibitors http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15167 Fri 19 Sep 2014 14:44:19 EST ]]> MEK-independent survival of B-RAFV600E melanoma cells selected for resistance to apoptosis induced by the RAF inhibitor PLX4720 http://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15166 V600E melanoma cells to B-RAF inhibitors. Experimental Design: B-RAFV600E melanoma cells were exposed to the B-RAF inhibitor PLX4720 for prolonged periods to select for cells resistant to apoptosis induced by the inhibitor. The resultant cells were analyzed for activation of extracellular signal regulated kinase (ERK), MAP/ERK kinase (MEK), and Akt, and related signals. Their roles in survival of the cells were also examined. Results: B-RAFV600E melanoma cells selected for resistant to PLX4720-induced apoptosis retained the V600E mutation in B-RAF, and proliferated steadily in the presence of the inhibitor, albeit with slow growth rate. These cells displayed high levels of ERK activation, that is, at least in part, independent of the conventional RAF/MEK/ERK pathway, as MEK activation was low and inhibition of MEK did not significantly block activation of ERK. In contrast, extracellular signals appeared involved. This was associated with elevated activation of the phosphoinositide 3-kinase (PI3k)/Akt pathway and could be inhibited by serum starvation and inhibition of PI3k/Akt. Inhibition of MEK did not impact on survival of these cells, whereas serum starvation, inhibition of PI3K/Akt, and inhibition of ERK1/2 reduced their viability. Conclusions: These results indicate that sensitivity to induction of apoptosis may be a major determinant of long-term responses of B-RAFV600E melanomas to specific inhibitors and suggest that rebound melanoma growth after initial treatment with the inhibitors may not be responsive to MEK inhibitors, but may be susceptible to inhibition of the PI3k/Akt pathway.]]> Fri 19 Sep 2014 14:37:20 EST ]]>