http://nova.newcastle.edu.au/vital/access/services/Feed ${session.getAttribute("locale")} 5 http://nova.newcastle.edu.au/vital/access/manager/Repository/uon:6564 Fertilization in mammalian eggs is accompanied by oscillatory changes in intracellular Ca²⁺ concentration, which are critical for initiating and completing egg activation events and the developmental program. Ca²⁺/Camodulin-dependent protein kinase II (CaMKII) is a multifunctional enzyme that is postulated to be the downstream transducer of the Ca²⁺ signal in many cell types. We tested the hypothesis that CaMKII is the major integrator of Ca²⁺-induced egg activation events and embryo development by microinjecting a cRNA that encodes a constitutively active (Ca²⁺-independent) mutant form of CaMKII (CA-CaMKII) into mouse eggs. Expression of this cRNA, which does not increase intracellular Ca²⁺, induced a sustained rise in CaMKII activity and triggered egg activation events, including cell cycle resumption, and degradation and recruitment of maternal mRNAs; cortical granule exocytosis, however, did not occur normally. Furthermore, when mouse eggs were injected with sperm devoid of Ca²⁺-releasing activity and activated with either CA-CaMKII cRNA or by SrCl₂,similar rates and incidence of development to the blastocyst stage were observed. These results strongly suggest that CaMKII is a major integrator of the Ca²⁺ changes that occur following fertilization. 2011-08-31T00:10:02.846Z ]]>