Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.13/917598

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Title
Cryopreservation-induced human sperm DNA damage is predominantly mediated by oxidative stress rather than apoptosis
Author/Creator
Thomson, L. K.;  Fleming, S. D.;  Aitken, R. J.;  De Iuliis, G. N.;  Zieschang, J. A.;  Clark, A. M.
Institution
The University of Newcastle. Faculty of Science & Information Technology, School of Environmental and Life Sciences
Description
Background: Whereas studies have revealed that the cryopreservation of human semen increases sperm DNA fragmentation, the mechanisms involved in this type of cryo-injury are largely unknown. Elucidation of these mechanisms may provide insight into preventing such injury. Methods: We obtained 60 semen samples from 60 men and conducted experiments to determine the cause of cryopreservation-induced DNA fragmentation using 8-oxo-7,8-dihydro-2'deoxyguanosine (8OHdG) as a biomarker of oxidative stress, percentage caspase positive cells as an indicator of apoptosis, the potential antioxidant genistein and the caspase inhibitor Z-VAD(OMe)-FMK. Results: Cryopreservation led to a significant increase in percentage DNA fragmentation, percentage 8OHdG and percentage caspase positive cells (P < 0.001). Percentage DNA fragmentation was positively correlated with percentage 8OHdG before (r = 0.756, P < 0.001) and after cryopreservation (r = 0.528, P = 0.017). The addition of 50 and 100 µM genistein to the cryoprotectant had a significant protective effect on sperm DNA (P < 0.001) although the caspase inhibitor demonstrated no difference to the control. Conclusions: Human sperm DNA fragmentation is associated with an increase in oxidative stress during cryopreservation, rather than the activation of caspases and apoptosis. The estrogenic compound genistein may be useful in reducing this effect but larger trials are needed to confirm this.
Relation
Human Reproduction Vol. 24, Issue 9, p. 2061-2070
Publisher Link
http://dx.doi.org/10.1093/humrep/dep214
Date
2009
Publisher
Oxford University Press
Keyword(s)
sperm DNA fragmentation;  cryopreservation;  8OHdG;  genistein caspase inhibitor
Resource Type
journal article
Identifier
http://hdl.handle.net/1959.13/917598
Identifier
ISSN:0268-1161
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Subject
"Human Reproduction"
 
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