Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.13/917555

OpenURL Link
Title
The structure of bacterial RNA polymerase in complex with the essential transcription elongation factor NusA
Author/Creator
Yang, Xiao;  Molimau, Seeseei;  Doherty, Geoffrey P.;  Johnston, Elecia B.;  Marles-Wright, Jon;  Rothnagel, Rosalba;  Hankamer, Ben;  Lewis, Richard J.;  Lewis, Peter J.
Institution
The University of Newcastle. Faculty of Science & Information Technology, School of Environmental and Life Sciences
Description
There are three stages of transcribing DNA into RNA. These stages are initiation, elongation and termination, and they are well-understood biochemically. However, despite the plethora of structural information made available on RNA polymerase in the last decade, little is available for RNA polymerase in complex with transcription elongation factors. To understand the mechanisms of transcriptional regulation, we describe the first structure, to our knowledge, for a bacterial RNA polymerase in complex with an essential transcription elongation factor. The resulting structure formed between the RNA polymerase and NusA from Bacillus subtilis provides important insights into the transition from an initiation complex to an elongation complex, and how NusA is able to modulate transcription elongation and termination.
Relation
EMBO Reports Vol. 10, Issue 9, p. 997-1002
Publisher Link
http://dx.doi.org/10.1038/embor.2009.155
Date
2009
Publisher
Nature Publishing Group
Keyword(s)
Bacillus subtilis;  NusA;  RNA polymerase;  single particle analysis;  transcription
Resource Type
journal article
Identifier
http://hdl.handle.net/1959.13/917555
Identifier
ISSN:1469-221X
Reviewed
Reviewed
4 Visitors 5 Hits 0 Downloads
Save/E-mail Citation
Citation Format
E-mail Address
Subject
"EMBO Reports"
 
OR