Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.13/917095

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Title
DNA damage in human spermatozoa is highly correlated with the efficiency of chromatin remodeling and the formation of 8-hydroxy-2′-deoxyguanosine, a marker of oxidative stress
Author/Creator
De Iuliis, Geoffry N.;  Thomson, Laura K.;  Mitchell, Lisa A.;  Finnie, Jane M.;  Koppers, Adam John;  Hedges, Andrew;  Nixon, Brett;  Aitken, R. John
Institution
The University of Newcastle. Faculty of Science & Information Technology, School of Environmental and Life Sciences
Description
DNA damage in human spermatozoa has been associated with a range of adverse clinical outcomes, including infertility, abortion, and disease in the offspring. We have advanced a two-step hypothesis to explain this damage involving impaired chromatin remodeling during spermiogenesis followed by a free radical attack to induce DNA strand breakage. The objective of the present study was to test this hypothesis by determining whether impaired chromatin protamination is correlated with oxidative base damage and DNA fragmentation in human spermatozoa. DNA fragmentation, chromatin protamination, mitochondrial membrane potential, and formation of the oxidative base adduct, 8-hydroxy-2′-deoxyguanosine (8OHdG), were monitored by flow cytometry/fluorescence microscopy. Impairment of DNA protamination during late spermatogenesis was highly correlated (P < 0.001) with DNA damage in human spermatozoa. The disruption of chromatin remodeling also was associated with a significant elevation in the levels of 8OHdG (P < 0.001), and the latter was itself highly correlated with DNA fragmentation (P < 0.001). The significance of oxidative stress in 8OHdG formation was demonstrated experimentally using H₂O₂/Fe2⁺ and by the correlation observed between this base adduct and superoxide generation (P < 0.001). That 8OHdG formation was inversely associated with mitochondrial membrane potential (P < 0.001) suggested a possible role for these organelles in the creation of oxidative stress. These results clearly highlight the importance of oxidative stress in the induction of sperm DNA damage and carry significant implications for the clinical management of this condition.
Relation
Biology of Reproduction Vol. 81, Issue 3, p. 517-524
Publisher Link
http://dx.doi.org/10.1095/biolreprod.109.076836
Date
2009
Publisher
Society for the Study of Reproduction
Keyword(s)
8OHdG;  chromatin protamination;  DNA damage;  spermatozoa;  superoxide
Resource Type
journal article
Identifier
http://hdl.handle.net/1959.13/917095
Identifier
ISSN:0006-3363
Reviewed
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Subject
"Biology of Reproduction"
 
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