Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.13/807034

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Title
Oncostatin M (OSM) is increased in asthma with incompletely reversible airflow obstruction
Author/Creator
Simpson, Jodie L.;  Baines, Katherine J.;  Boyle, Michael J.;  Scott, Rodney J.;  Gibson, Peter G.
Institution
The University of Newcastle. Faculty of Health, School of Medicine and Public Health
Description
Oncostatin M, a unique member of the interleukin (IL)-6 cytokine family, is thought to be involved in airway remodeling. The expression of oncostatin M in the lower airways is unknown. The aim of this study was to measure the sputum expression of oncostatin M in patients with asthma with and without irreversible airflow obstruction. Induced sputum was collected from nonsmoking adults with stable asthma (n = 53), 31 with incomplete reversibility of airflow obstruction. Peripheral blood cells were isolated and stimulated with lipopolysaccharide in 10 participants with asthma and irreversible airflow obstruction. Oncostatin M protein levels were determined in supernatant, whereas RNA was extracted to determine Oncostatin M mRNA expression using real-time polymerase chain reaction (PCR). Oncostatin M mRNA expression and protein levels were significantly higher in the sputum of asthmatics with irreversible airflow obstruction. Sputum oncostatin M levels were highest in people with severe airflow obstruction and were localized to airway neutrophils and macrophages. Peripheral blood neutrophils released more oncostatin M when stimulated with lipopolysaccharide compared with unstimulated neutrophils. Sputum oncostatin M is increased in asthma with irreversible airflow obstruction and is present in airway neutrophils and macrophages. Oncostatin M may link airway inflammation to remodeling in asthma.
Relation
Experimental Lung Research Vol. 35, Issue 9, p. 781-794
Publisher Link
http://dx.doi.org/10.3109/01902140902906412
Date
2009
Publisher
Informa Healthcare
Keyword(s)
airflow obstruction;  asthma;  IL-6 family;  induced sputum;  oncostatin M
Resource Type
journal article
Identifier
http://hdl.handle.net/1959.13/807034
Identifier
ISSN:0190-2148
Reviewed
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Subject
"Experimental Lung Research"
 
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