Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.13/808157
- Title
- Actin filament dynamics are dominated by rapid growth and severing activity in the Arabidopsis cortical array
- Author/Creator
-
Staiger, Christopher J.;
Sheahan, Michael B.;
Khurana, Parul;
Wang, Xia;
McCurdy, David W.;
Blanchoin, Laurent
- Institution
- The University of Newcastle. Faculty of Science & Information Technology, School of Environmental and Life Sciences
- Description
- Metazoan cells harness the power of actin dynamics to create cytoskeletal arrays that stimulate protrusions and drive intracellular organelle movements. In plant cells, the actin cytoskeleton is understood to participate in cell elongation; however, a detailed description and molecular mechanism(s) underpinning filament nucleation, growth, and turnover are lacking. Here, we use variable-angle epifluorescence microscopy (VAEM) to examine the organization and dynamics of the cortical cytoskeleton in growing and nongrowing epidermal cells. One population of filaments in the cortical array, which most likely represent single actin filaments, is randomly oriented and highly dynamic. These filaments grow at rates of 1.7 μm/s, but are generally short-lived. Instead of depolymerization at their ends, actin filaments are disassemble by severing activity. Remodeling of the cortical actin array also features filament buckling and straightening events. These observations indicate a mechanism inconsistent with treadmilling. Instead, cortical actin filament dynamics resemble the stochastic dynamics of an in vitro biomimetic system for actin assembly.
- Relation
- Journal of Cell Biology Vol. 184, Issue 2, p. 269-280
- Publisher Link
- http://dx.doi.org/10.1083/jcb.200806185
- Date
- 2009
- Publisher
- Rockefeller University Press
- Keyword(s)
-
actin filaments;
Arabidopsis;
cortical array;
variable-angle epifluorescence microscopy
- Resource Type
- journal article
- Identifier
- http://hdl.handle.net/1959.13/808157
- Identifier
- ISSN:0021-9525
- Reviewed
- Full Text
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