Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.13/804311

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Title
Unique protein kinase C profile in mouse oocytes: lack of calcium-dependent conventional isoforms suggested by rtPCR and Western blotting
Author/Creator
Gangeswaran, Rathi;  Jones, Keith T.
Description
rtPCR and Western blotting were used to determine which members of the PKC family are present in both immature and mature mouse oocytes. Using isoform-specific PCR primers and antibodies PKC-δ and -λ were detected while such techniques failed to observe the conventional isoforms of PKC-α, -β, -γ. This isoform profile was confirmed using an alternative PCR strategy, which allowed discrimination of PCR products derived from conventional and novel PKC isoforms. In addition PKC-ε, -η, -θ and -ζ were not detected by rtPCR. These results suggest that the predominant isoforms in oocytes are PKC-δ and -λ.
Relation
FEBS Letters Vol. 412, Issue 2, p. 309-312
Publisher Link
http://dx.doi.org/10.1016/S0014-5793(97)00782-5
Date
1997
Publisher
Elsevier BV
Keyword(s)
protein kinase C;  oocytes;  calcium;  fertilization;  mouse;  PCR
Resource Type
journal article
Identifier
http://hdl.handle.net/1959.13/804311
Identifier
ISSN:0014-5793
Language
eng
Reviewed
Reviewed
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Subject
"FEBS Letters"
 
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