Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.13/804299

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Title
A mammalian sperm cytosolic phospholipase C activity generates inositol trisphosphate and causes Ca²⁺ release in sea urchin egg homogenates
Author/Creator
Jones, Keith T.;  Cruttwell, Caroline;  Parrington, John;  Swann, Swann
Description
Injection of sperm extracts triggers Ca²⁺ oscillations in mammalian eggs similar to those seen at fertilisation. Here, we show that addition of sperm extracts to sea urchin egg homogenates causes Ca²⁺ release and inositol 1,4,5-trisphosphate (InsP₃) production. Furthermore depleting homogenates of phosphatidylinositol lipids using a phosphatidylinositol-specific phospholipase C blocked the sperm extract from causing InsP₃ production and a Ca²⁺ rise. A response could be recovered by the addition of phosphatidylinositol 4,5-bisphosphate to either sperm extracts or egg homogenates. These data indicate that sperm extracts contain an InsP₃-generating phospholipase C which may play a role in Ca²⁺ release at fertilisation.
Relation
FEBS Letters Vol. 437, Issue 3, p. 297-300
Publisher Link
http://dx.doi.org/10.1016/S0014-5793(98)01254-X
Date
1998
Publisher
Elsevier BV
Keyword(s)
egg;  sperm;  calcium;  inositol trisphosphate;  phospholipase C
Resource Type
journal article
Identifier
http://hdl.handle.net/1959.13/804299
Identifier
ISSN:0014-5793
Language
eng
Reviewed
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Subject
"FEBS Letters"
 
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