Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.13/804296

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Title
The soluble sperm factor that causes Ca²⁺ release from sea-urchin (Lytechinus pictus) egg homogenates also triggers Ca²⁺ oscillations after injection into mouse eggs
Author/Creator
Parrington, John;  Jones, Keith T.;  Lai, F. Anthony;  Swann, Karl
Description
Cytosolic extracts of boar sperm contain a soluble phospholipase C (PLC) activity that induces Ca²⁺ release in sea-urchin (Lytechinus pictus) egg homogenates and an uncharacterized protein factor that causes Ca²⁺ oscillations when injected into mammalian eggs. In the present study we fractionated boar sperm extracts on three different FPLC chromatographic columns and found that the fractions that caused maximal Ca²⁺ release in sea-urchin egg homogenates were also the ones that triggered Ca²⁺ oscillations in mouse eggs. Our data suggests that the sperm factor which triggers Ca²⁺ oscillations in eggs contains a PLC and not the 33 kDa glucosamine deaminase previously suggested to be one its components.
Relation
Biochemical Journal Vol. 341, Issue 1, p. 1-4
Relation
http://www.biochemj.org/bj/341/bj3410001.htm
Date
1999
Publisher
Portland Press
Keyword(s)
cytosolic;  phospholipase C;  calcium;  egg homogenates;  sperm;  oscillations
Resource Type
journal article
Identifier
http://hdl.handle.net/1959.13/804296
Identifier
ISSN:0264-6021
Language
eng
Reviewed
Reviewed
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Subject
"Biochemical Journal"
 
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